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Fibrinogen Structural Changes Caused by Glass Surface Adsorption Detected by Solid Surface Fluorescence Spectroscopy

Fibrinogen Structural Changes Caused by Glass Surface Adsorption Detected by Solid Surface Fluorescence Spectroscopy *
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摘要 Solid surface fluorescence (SSF) spectra of fibrinogen (FG) adsorbed on a glass surface were measured. For comparison, solution fluorescence (SF) spectra of native, thermally denatured, urea denatured FG solution with FG desorbed from a glass surface and the SSF spectra of native FG powder were recorded. To analyze the structural changes induced by the surface adsorption, all the aqueous samples mentioned above were analyzed by circular dichroism (CD) spectroscopy. The results demonstrated that the structural changes of FG induced by glass adsorption caused changes in the spectroscopic features of the SSF spectra, especially in the excitation (EX) spectra. It is suggested that SSF spectroscopy may be a useful tool for studying the structures of surface/interface biomolecules and for evaluating the biocompatibility of biomaterials. Solid surface fluorescence (SSF) spectra of fibrinogen (FG) adsorbed on a glass surface were measured. For comparison, solution fluorescence (SF) spectra of native, thermally denatured, urea denatured FG solution with FG desorbed from a glass surface and the SSF spectra of native FG powder were recorded. To analyze the structural changes induced by the surface adsorption, all the aqueous samples mentioned above were analyzed by circular dichroism (CD) spectroscopy. The results demonstrated that the structural changes of FG induced by glass adsorption caused changes in the spectroscopic features of the SSF spectra, especially in the excitation (EX) spectra. It is suggested that SSF spectroscopy may be a useful tool for studying the structures of surface/interface biomolecules and for evaluating the biocompatibility of biomaterials.
出处 《Tsinghua Science and Technology》 SCIE EI CAS 1997年第1期42-47,共6页 清华大学学报(自然科学版(英文版)
关键词 solid surface fluorescence (SSF) spectroscopy glass surface adsorption fibrinogen (FG) structure of surface/interface biomolecules solid surface fluorescence (SSF) spectroscopy glass surface adsorption fibrinogen (FG) structure of surface/interface biomolecules
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