摘要
本文用神经丝（ＮＦ）免疫组织化学方法在１５例人体尸检材料中研究了脊髓创伤后生存２ｈ～９Ｗ的脊髓神经元胞体和轴突的病理学改变。结果表明：脊髓创伤后２ｈ，神经丝免疫组织化学反应即可显示ＮＦ阳性反应产物在轴突内聚集。创伤后第４天，病变的前角运动神经元胞体内神经丝反应异常地增强。以上结果表明：神经丝免疫组织化学方法比常规显示轴突的染色方法能更早更清晰地显示脊髓内轴突的病变，并进一步证实了创伤后细胞骨架紊乱在神经元的病理发病机理中起重要作用。
The pathological changes of cell bodies and a-cons of motorneurons were investigatedwith the immunohistochemical method for neurofilament(NF)on autopay meterials of human traumatic injuried spinal cord obtained from 15 patients.Their survival period rangedfrom 2 hours to 9 weeks.The results were as follows;2 hours after injury, the swollen a-cons could be shown by NF immunohistochemical reaction.On the fourth day after injury,the excessive misaccumlation of NF was observed in the cell bodies and a-cons of motorneurons in ventral horn. The above results proved that after trauma,the disorder of cytoskeleton played an important role in the pathogenesis of neuron's cell body and a-cons. The pathological changes of axon were demonstrated earlier and more clearly by NF immunohistochemical reaction than by ordinary stain methods for axon.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1995年第2期134-138,共5页
Chinese Journal of Histochemistry and Cytochemistry
关键词
神经丝
免疫组织化学
人脊髓创伤
Neurofilament immunohistochemy
human spinal cord trauma.
