摘要
AbstractThe polymerase chain reaction (PCR) technique, compared with dot-blot DNA hybridization, was used to detect E6 transformation gene ofhuman papillomavirus type 16, in the tissues of 10oral malignant tumors. The tumors were: 6 oralmucosal squamous cell carcinomas (SCC), 2 salivary glandular adenoid cystic carcinomas (ACC),1 malignant pleomorphic adenoma (MPA ), and 1embryoid rhabdomysarcoma (ER ) in the soft tissue of the palate, 10 non-tumor cases including 5cleft lips and 5 cleft palates were chosen for control. The results with the two techniques were basically identical. 6 out of the 10(6/10 ) tumors,which were 4 SCC, 1 ACC and 1 ER, were positive for the E6 transformation gene. In the nontumor control, none of the 4 (0/4) cases detectedwith dot-blot DNA hybridization showed a definitepositive result, except for 1 cleft lip with a weaklypositive result. All the 10 non-tumor cases detected with PCR showed negtive results (0/10). Thedifference of the positive rate between the twogroups was significant (P < 0. 05 ). The resultsprovided data for the study on HPV virologicaloncogenesis of the oral cancers.
AbstractThe polymerase chain reaction (PCR) technique, compared with dot-blot DNA hybridization, was used to detect E6 transformation gene ofhuman papillomavirus type 16, in the tissues of 10oral malignant tumors. The tumors were: 6 oralmucosal squamous cell carcinomas (SCC), 2 salivary glandular adenoid cystic carcinomas (ACC),1 malignant pleomorphic adenoma (MPA ), and 1embryoid rhabdomysarcoma (ER ) in the soft tissue of the palate, 10 non-tumor cases including 5cleft lips and 5 cleft palates were chosen for control. The results with the two techniques were basically identical. 6 out of the 10(6/10 ) tumors,which were 4 SCC, 1 ACC and 1 ER, were positive for the E6 transformation gene. In the nontumor control, none of the 4 (0/4) cases detectedwith dot-blot DNA hybridization showed a definitepositive result, except for 1 cleft lip with a weaklypositive result. All the 10 non-tumor cases detected with PCR showed negtive results (0/10). Thedifference of the positive rate between the twogroups was significant (P < 0. 05 ). The resultsprovided data for the study on HPV virologicaloncogenesis of the oral cancers.
