摘要
A new imidazolate bridged Cu^(2+),Zn^(2+)binuclear complex[(dtma) CulmZn (dtma)]ClO_4· 2.5H_2O taken as active site model for Cu,Zn-SOD has been synthesized and its crystal structure determined.All the bond lengths, bond angles and the distance between Cu and Zn atoms in Cu-Im- Zn core of the model complex are close to those in Cu,Zn-SOD.ESR parameters of the model complex as a function of pH show that the imidazolate bridge is stable in pH range 10—12,and is broken on Zn side at pH～9.With decreasing pH,the imidazole is released at pH～4 and the dtma ligand dissociates from the Cu containing fragment at pH～2.4.
A new imidazolate bridged Cu^(2+),Zn^(2+)binuclear complex[(dtma) CulmZn (dtma)]ClO_4· 2.5H_2O taken as active site model for Cu,Zn-SOD has been synthesized and its crystal structure determined.All the bond lengths, bond angles and the distance between Cu and Zn atoms in Cu-Im- Zn core of the model complex are close to those in Cu,Zn-SOD.ESR parameters of the model complex as a function of pH show that the imidazolate bridge is stable in pH range 10—12,and is broken on Zn side at pH～9.With decreasing pH,the imidazole is released at pH～4 and the dtma ligand dissociates from the Cu containing fragment at pH～2.4.
