摘要
大肠癌是严重危害人类健康的恶性肿瘤之一,早期在临床尚无症状出现前就可有大便潜血试验阳性,提高阳性检出率是目前研究的重要内容。本文报告了应用高度特异的抗人血红蛋白单克隆抗体研究成果,采用斑点酶免疫吸附试验(Dot—ELISA)。检测人粪便中潜血,研究了该法的敏感性、特异性和适用性。结果表明该法使用的纤维素膜有极强的吸附力,提高了检测灵敏度。该抗体只与人血红蛋白反应。对9421例正常人的粪便进行了检查,发现早期大肠癌患者5例。研究表明该法可作为早期大肠癌普查初筛手段,对提高大肠癌治愈率和生存率有积极作用,是一项值得推广的新技术。
Colonic carcinoma is one kind of the malignant tumors which seriously jeopardize the human being, Positive occult blood in stool may appear long before the clinical evidences of the disease. One of the important research for the diagnosis of the disease has been used to increase the detectable rate of the test. In this study, Dot Engyme Linked Immunosorbent Assay (Dot--ELISA), a technique using the highly specific anti-human- hemoglobin monoclonal antitody, was applied to detect the occult blood in stool. The sensitivity, specificity and the applicability of the technigue were studied o The cellulose film, used in this technique was proved to have a very strong absorptive capacity. This antibody reacted only with human hemoglobin. A high detectable rate was found. Five cases with colonic carcinoma were defected from 9421 subjects examined with this technique. The result indicated that the Dot--ELISA technique could be used to screen the colonic cancer in early stage. It is suggested that this new technique deserve to be spreaded.
出处
《西南国防医药》
CAS
1992年第6期336-337,389,共3页
Medical Journal of National Defending Forces in Southwest China
基金
全军"七五"攻关招标
关键词
单克隆抗体
斑点醇免疫吸附试验
潜血
monoclonal antibody, dot engyme linked immunosorbent assay, occult blood
