摘要
目的:探讨维甲酸致大鼠骨质疏松对下颌骨骨量的影响。方法:20只3月龄SD雌性大鼠,按随机数字表法分为对照组和模型组,每组10只。采用维甲酸灌胃建立大鼠骨质疏松模型以诱发下颌骨骨丢失,通过下颌骨骨密度测量;血中各项生化指标的检测;下颌骨解剖形态观察以及骨组织形态计量法测量等方法,观测维甲酸诱导的模型对下颌骨骨量的影响。结果:灌胃15d后,立体显微镜下可见,模型组大鼠下颌角下缘和下颌孔上缘有骨膜下骨吸收区存在;模型组大鼠下颌骨磨牙段骨密度为(0.281±0.017)g/cm2,比对照组骨密度犤(0.324±0.005)g/cm2犦明显降低(t=5.944,P<0.05);骨组织形态计量法结果显示,模型组下颌骨骨小梁平均宽度显著低于对照组(t=26.302,P<0.05);模型组下颌骨骨小梁平均间隔宽度显著高于对照组(t=19.963,P<0.05)。与对照组相比,血清雌二醇水平显著降低,血清碱性磷酸酶和抗酒石酸酸性磷酸酶活性显著升高。结论:灌服维甲酸后,颌骨出现了疏松化改变,表明该模型用于研究下颌骨骨丢失是可行的。
AIM: To explore the impact of osteoporosis induced by retinoic acid on bone mass of mandible in rats. METHODS: Twenty SD female rats aged three months were randomly allocated into normal group(n=10) and model group(n=10).Osteoporosis model was established by retinoic acid through stomach perfusion in rats to induce mandibular bone loss.The impact of model induced by retinoic acid on mandibular bone mass was observed through the detection of bone mineral density and each serous biochemical index and the observation of the anatomic morphology of mandible as well as bone histomorphological metrological measurements, etc. RESULTS:After 15 day of the administration of retinoic acid,bone resorption regions in subperiosteum were seen in the lower border of mandibular angle and the upper border of mandibular foramen in the rats of model group under anatomic microscope.The bone mineral density of mandibular molar part in the rats of model group was(0.281±0.017)g/cm2,which was significantly lower compared with(0.324±0.005)g/cm2 of control group(t=5.944,P< 0.05). The trabecular width in model group was significantly lower than that of control group(t=26.302,P< 0.05).The separation of trabecular in model group was significantly higher than that of control group(t=19.963,P< 0.05). Compared with control group,the serous level of estradiol significantly decreased,the activity of serous alkaline phosphatase(ALP) and tartrate resistant acid phosphatase(TRAP) was markedly increased in model group. CONCLUSION: Bone mass of mandible decreased in rats with osteoporosis induced by retinoic acid through stomach perfusion, which indicates that this model is available and feasible in the study of mandibular bone loss.
出处
《中国临床康复》
CAS
CSCD
2004年第18期3684-3685,共2页
Chinese Journal of Clinical Rehabilitation
