摘要
本文报告通过超低温(—196℃)肿瘤动物保存的方法,详细观察了六种动物肿瘤细胞的生物学特性。其结果是:冷冻保存瘤鼠31只,时间4—310d,经复温后共移植于健康 NIH 小鼠183只,移植后于6—8d 发病172只,移植成功率93.98%。其中:EAC 瘤株冷冻保存5只,复温后移植于健康 NIH 鼠29只,于6—8d 均发病;S180瘤株,冷冻保存12只,复温后移植于健康 NIH鼠72只,于7—9d 发病;HAC 瘤株冷冻保存7只,复苏后移植于健康 NIH 鼠32只,于7—9d 均发病;ARS 瘤株冷冻保存3只,复苏后移植于健康 NIH 鼠20只,于7—9d 均发病;L615瘤株,冷冻保存2只,复苏后移植于健康 NIH 鼠15只,于8d 均发病;S37瘤株冷冻保存2只,复苏后移植于健康 NIH 鼠15只,于8d 后发病4只。除此株制取瘤细胞悬液不当造成移植成功率低外,其他5种瘤株均获100%移植成功。此法的优点是:超低温保存瘤鼠即是一个“瘤细胞库”,可以反复冷冻复温存取转种,并获100%成功,打破了前人“瘤细胞液氮内一次存取论”。保存瘤细胞量大,作为一个瘤细胞库,方法简便,节约时间,冷冻全过程不超过20min,比单细胞冷冻保存节约时间2—5倍,节约人力物力财力。
Using ultra-hypothermic(-196℃)preservation of the whole tumor mice,authors of this paper reported results of the biological characteristics of the tumor cells in six different strains.25mice were stored under ultra-low temperature for 4-120 days.After re- turning to normothermia,transplantation was made in 146 normal mice(NIH),in which 137 mice were fallen sick after a period of 6- 8 days,with a successful rate amounting to 93.84%.Detailed in- formation may be given as follows. (1)EAC-strain:3 mice of this strain were cold stored and 20 normal mice were transplanted after returning to normothermia,all were fallen sick within 6-8 days;(2)s180-strain:11 mice of this strain were cold stored and transplantation was made in 67 normal mice after returning to normothermia,all were fallen sick within 7- 9 days;(3)HAC-strain:6 mice of this strain were cold stored and 29normal mice were transplanted,all were fallen sick within 7-9 days;(4)ARS-strain:3 mice of this strain were cold stored and 29 normal mice were transplanted,all were fallen sick within 7-9 days; (5)L615-strain:2 mice of this strain were cold stored and trans- plantation was made in 10 normal mice,all were fallen sick in 8 days;(6)S37-strain:2 mice of this strain were cold stored and transplantation was made in 10 normal mice,but only one was fall- en sick.The cause of this failure may be attributed to improper preparation of the suspension.Aside from this exception,our suc- cessful rate may be accounted for 100%. The advantages of the ultra-hypothermic preservation are man- ifold.(1)It can be used as a tumor cell storage.The preserved and resuscitated mice may be transplanted repeatedly with 100% suc- cess.The theory that“tumor cells can be preserved and used only once”is chanllenged.(2)Since it may be used as a storage,great quantities of tumor cells can be preserved.(3)This method is simple and frugal of time.The whole freezing process may be completed within 20 minutes.Two to five-fold of time may be saved than the formerly used“only once”method.In addition,it saves a lot of man- power,material and instrument.No preservatives and nutrients are needed.It is 95% economic financially. Our success is based mainly on the following reasons.In the present way of preservation,the host itself,after freezing,may be served as a protective body and may be able to supply metabolites to the tumor cells even under ultra-hypothermic conditions,so that the viability of these cells still exists.A safety environmemt for living is mantained for the tumor cells in the host,and their reproductive ca- pacity may be strengthened.
出处
《中国卫生检验杂志》
CAS
1992年第1期18-21,共4页
Chinese Journal of Health Laboratory Technology
