摘要
目的 观察罗格列酮对2型糖尿病大鼠肾皮质趋化因子fractalkine表达的影响.方法 SD大鼠30只,10只分至正常对照组,20只腹腔注射小剂量链脲佐菌素结合高能量饲料制备2型糖尿病大鼠模型,然后分至糖尿病组(n=10)及罗格列酮干预组(n=10).运用流动注射分析法测定血清糖基化终产物-肽(advanced glycosylation end products-peptide,AGE-P),应用半定量逆转录聚合酶链反应和免疫组织化学法分别检测肾皮质fractalkine mRNA和蛋白表达.选用单因素方差分析进行数据统计分析.结果 糖尿病组大鼠血清AGE-P[(2.87±0.21)U/ml]、肾皮质fractalkine mRNA(1.41±0.03)及fractalkine蛋白表达(0.79±0.04)均明显高于对照组[分别为(0.90±0.13)U/ml、0.85±0.04及0.46±0.03,P<0.01];罗格列酮干预组大鼠血清AGE-P[(1.45±0.15)U/ml]、肾皮质fractalkine mRNA(1.00±0.05)及fractalkine蛋白表达(0.67±0.03)则明显低于糖尿病组(P<0.01).结论 罗格列酮可能通过抑制肾皮质fractalkine表达发挥其肾脏保护作用.
Objective To investigate the effect of rosiglitazone on the expression of fractalkine in renal cortex of type 2 diabetic rats. Methods Thirty Sprague-Dawley (SD)rats were randomly divided into normal control group (n=10) and type 2 diabetic model groups which were induced by intraperitoneal injection of low-dose streptozotocin combined with high fat diet. After diabetes established, diabetic rats were then randomized to receive rosiglitazone (n = 10) or vehicle (n=10) treatment. Serum advanced glycosylation end products-peptide (AGE-P) was measured by using flow injection assay. The mRNA and protein expressions of fractalkine were detected by semi-quantitative reverse transcription polymerase chain reaction and immunohistochemical staining, respectively. One-way analysis of variances was used for data analysis. Results The serum AGE-P [(2.87±0.21) U/ml], and the expression of fractalkine mRNA (1.41±0. 03) and protein (0. 79±0.04) in the renal cortex were significantly higher in the diabetes group than the normal control group [(0.90±0.13) U/ml, 0.85±0.04, and 0.46±0.03; P<0.01].However, the serum AGE-P [(1.45±0.15) U/ml], and the expression of fractalkine mRNA (1.00± 0. 05) and protein (0. 67±0.03) in the rosiglitazone group were significantly lower compared to the diabetes group (P < 0.01). Conclusions Rosiglitazone may have renoprotective effects via inhibiting fractalkine expression in the renal cortex.
出处
《中华糖尿病杂志》
CAS
2009年第2期-,共4页
CHINESE JOURNAL OF DIABETES MELLITUS
基金
教育部留学回国人员科研启动基金，无锡市科技基金
