摘要
茶叶中的生物碱具有许多独特的生理活性,主要含有可可碱、茶碱和咖啡碱,也有少量嘧啶类生物碱.采用反相高效液相色谱法,在优化的色谱条件下,建立了一个同时分离测定可可碱、茶碱和咖啡碱的反相高效液相色谱法,快速准确地测定这些生物碱在古树茶叶中的含量,对评估古树茶的品质和其保健功效有着重要的参考价值.
Alkaloids from tea have many unique physiological activities,mainly containing theobromine theophylline and caffeine,there are a small number of pyrimidine alkaloids as well.A new reversed-phase-high performance liquid chromatographic procedure for the determination of alkaloids was described.The method has been used for separation and determination of the theobromine,theophylline and caffeine in tea and tea drinking.The results showed the method,which is simple,rapid,sensitive and of wide linear range,could be used for the determination of alkaloids in tea and be assessed the quality of ancient teas and its health function.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第S2期339-343,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南大学2010年立项校级本科生科研项目-普洱茶发酵菌类对嘌呤碱含量的影响研究
关键词
茶
生物碱
可可碱
茶碱
咖啡碱
检测
tea
alkaloids
theobromine
theophylline
caffeine
determination
