摘要
应用EST-SSR标记技术对云南省不同区域采集的39份古茶树资源进行了遗传多样性和亲缘关系研究。结果表明:43对EST-SSR引物共扩增出121条多态性谱带,有效等位基因变异占比重的73.88%。多态性信息量PIC值为0.05~0.74,平均为0.469。基因多样性指数平均值为0.53,Shannon指数平均值为0.81,均高于0.5。遗传相似系数0.41~0.91,平均值为0.65,显示云南大茶树的遗传基础相对比较宽,具有较高的遗传多样性水平。SSR聚类分析表明,大茶树的遗传距离和亲缘关系与地理分布有一定的关系,在分子水平上显示了部分云南大茶树的亲缘关系,有助于今后野生大茶树的保护及利用研究。
The genetic diversity and relationship of 39 ancient tea varieties gathered in different areas in Yunnan were analyzed by EST-SSR markers. The results showed that total 121 alleles were amplified,and effective number of alleles was 73. 88%. The polymorphism information content( PIC) was ranging from 0. 05 to 0. 74 with the average of 0. 469. The average genetic diversity and the average Shannon's information index were 0. 55 and 0. 81 respectively,which was higher than 0. 5. The Nei's original measures of genetic identity among those tea germplasms ranged from 0. 441 to 0. 907,with average of 0. 653,and this results indicated that the genetic basis among those tea germplasms was wide,and the level of genetic diversity within those ancient tea varieties was high. The cluster analysis based on SSR markers revealed that the genetic distance and the genetic relationship within most of those varieties were related by its geographic source. The genetic relationship of all 39 ancient tea plants revealed by SSR dendrogram would be greatly helpful for the protection and utilization research on wild ancient tea varieties in the future.
出处
《华北农学报》
CSCD
北大核心
2013年第S1期91-96,共6页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31160175)
农业部农作物种质资源保护与利用项目(NB2012-2130135)
云南省科技创新人才计划项目(2011CI068)
国家茶叶现代产业技术体系建设项目(NYCYTX-23)
云南省农业科学院院专项(YAAS2012ZY002)
