期刊文献+

Gene expression profiles in peripheral blood mononuclear cells of ulcerative colitis patients 被引量:1

Gene expression profiles in peripheral blood mononuclear cells of ulcerative colitis patients
下载PDF
导出
摘要 AIM: To identify peripheral blood mononuclear cell (PBMC) gene expression profiles of ulcerative colitis (UC) patients, using oligonucleotide microarrays, to gain insights into UC molecular mechanisms. METHODS: The Human OneArray microarrays were used for a complete genome-wide transcript profiling of PBMCs from 12 UC patients and 6 controls. Differential analysis per gene was performed with a random variance model; t test and P values were adjusted to control the false discovery rate (5%). Gene ontology (GO) was deployed to analyze differentially expressed genes at significant levels between patients and controls to identify the biological processes involved in UC. RESULTS: Comparative analysis revealed that 4438 probes (4188 genes) were differentially expressed between the two groups, of which 3689 probes (3590 genes) were down-regulated whereas 749 probes (598 genes) were up-regulated. Many disregulated genes in our data have been reported by previous microarray studies carried out on intestinal mucosa samples, such as S100A8 , CEACAM1 and S100A9 . GO enrichment analysis revealed 67 high enrichment up-regulated categories and one significant down-regulated category. The up-regulated genes were mainly involved in immune and inflammatory response, cell cycle and proliferation, DNA metabolism and repair. CONCLUSION: Gene expression profiling of PBMCs from patients with UC has highlighted several novel gene categories that could contribute to the pathogenesis of UC. AIM: To identify peripheral blood mononuclear cell (PBMC) gene expression profiles of ulcerative colitis (UC) patients, using oligonucleotide microarrays, to gain insights into UC molecular mechanisms. METHODS: The Human OneArray microarrays were used for a complete genome-wide transcript profiling of PBMCs from 12 UC patients and 6 controls. Differential analysis per gene was performed with a random variance model; t test and P values were adjusted to control the false discovery rate (5%). Gene ontology (GO) was deployed to analyze differentially expressed genes at significant levels between patients and controls to identify the biological processes involved in UC. RESULTS: Comparative analysis revealed that 4438 probes (4188 genes) were differentially expressed between the two groups, of which 3689 probes (3590 genes) were down-regulated whereas 749 probes (598 genes) were up-regulated. Many disregulated genes in our data have been reported by previous microarray studies carried out on intestinal mucosa samples, such as S100A8, CEACAM1 and S100A9. GO enrichment analysis revealed 67 high enrichment up-regulated categories and one significant down-regulated category. The up-regulated genes were mainly involved in immune and inflammatory response, cell cycle and proliferation, DNA metabolism and repair. CONCLUSION: Gene expression profiling of PBMCs from patients with UC has highlighted several novel gene categories that could contribute to the pathogenesis of UC.
出处 《World Journal of Gastroenterology》 SCIE CAS 2013年第21期3339-3346,共8页 世界胃肠病学杂志(英文版)
基金 Supported by Grants from National Natural Science Foundation of China, No. 81260074/H0310 and 81160055/H0310 Confederative Special Foundation of Science and Technology, Department of Yunnan Province and Kunming Medical College, No.2011FB183 and 2007C0010R Medical Academic Leader of Yunnan Provincial Bureau of Health, No. D-201215
关键词 ULCERATIVE COLITIS MICROARRAY Gene ontology Peripheral blood MONONUCLEAR cells Ulcerative colitis Microarray Gene ontology Peripheral blood mononuclear cells
  • 相关文献

参考文献11

  • 1Chris Stevens MD,Gerd Walz MD,Chander Singaram MD,Mark L. Lipman MD,Bernd Zanker MD,Aldo Muggia MD,Donald Antonioli MD,Mark A. Peppercorn MD,Terry B. Strom MD.Tumor necrosis factor-α, interleukin-1β, and interleukin-6 expression in inflammatory bowel disease[J].Digestive Diseases and Sciences.1992(6)
  • 2Ouyang Q,Tandon R,Goh K L,et al.The emergence of inflammatory bowel disease in the Asian Pacific region[].Current Opinion.2005
  • 3Dieckgraefe BK,Stenson WF,Korzenik JR,et al.Analysis of mucosal gene expression in inflammatory bowel disease by parallel oligonucleotide arrays[].Physiological Genomics.2000
  • 4Lawrance IC,Fiocchi C,Chakravarti S.Ulcerative colitis and Crohn’s disease: distinctive gene expression profiles and novel susceptibility candidate genes[].Human Molecular Genetics.2001
  • 5Kornbluth A,Sachar DB.Ulcerative colitis practice guidelines in adults (update): American College of Gastroenterology, Practice Parameters Committee[].The American journal of Gastroenterology.2004
  • 6Engene C,Butcher.Leukocyte-endothelial cell recognition: three steps to specificity and diversity[].Cell.1991
  • 7Guo,HH,Loeb,LA.Tumbling down a different pathway to genetic instability[].The Journal of Clinical Investigation.2003
  • 8NA Braus,DE Elliott.Advances in the pathogenesis and treatment of IBD[].Clinical Immunology.2009
  • 9Colliver DW,Crawford NP,Eichenberger MR,et al.Molecularprofiling of ulcerative colitis-associated neoplastic progression[].Experimental and Molecular Pathology.2006
  • 10C. M. Costello,N. Mah,R. Hasler,P. Rosenstiel,G. H. Waetzig,A. Hahn,T. Lu,Y. Gurbuz,S. Nikolaus,M. Albrecht,J. Hampe,R. Lucius,G. Kloppel,H. Eickhoff,H. Lehrach,T. Lengauer,S. Schreiber.Dissection of the inflammatory bowel disease transcriptome using genome-wide cDNA microarrays[].PLoS Med.2005

同被引文献4

引证文献1

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部