摘要
利用组织培养结合秋水仙素对滇杨进行多倍体诱导,以期获得滇杨多倍体植株。结果表明:组织培养结合秋水仙素诱导滇杨产生多倍体的较优组合为秋水仙素浓度为80 mg/L下处理30 d或在90 mg/L下处理20～30 d,此时,多倍化诱导率最高可达18.6%。对滇杨多倍体植株与正常植株进行外部形态、染色体数目及单位叶面积内气孔数目的观察,发现多倍体植株与正常株形态差异明显,染色体数目增加为76条,叶表皮气孔变大,同时,叶片中叶绿素含量约为二倍体的1.5倍。
Populus yunnanensis Dode was employed to induce polyploid by tissue Culture combined with colchicine in order to obtain new polyploid P.yunnanensis Dode.The result showed that the better combinations were 80 mg/L colchicine treated for 30 days or 90 mg/L colchicum treated for 20-30 days during tissue culture,and gotten the highest inducing rate was 18.6%.The morphological difference,chromosome number,and stomas size were also observed between normal and various P.yunnanensis.The morphological difference was very clearer between normal and variant P.yunnanensis.The chromosome number increased to 76,and the size of stomas increased in variant P.yunnanensis.The chlorophyll content in leaves was 1.5 times higher in variant P.yunnanensis than that in normal plants.
出处
《云南农业大学学报》
CAS
CSCD
北大核心
2013年第2期251-256,共6页
Journal of Yunnan Agricultural University
基金
云南省教育厅科学研究基金重点项目(2012Z066)
云南省应用基础研究基金资助项目(2009CD071)
国家自然基金项目(30960320)
西南林业大学校级重点学科林木遗传育种学(XKX200904)
关键词
滇杨
组织培养
秋水仙素
多倍体
诱导
Populus yunnanensis Dode
tissue culture
colchicine
polyploid
inducement
