摘要
本文分别采集鸭胚与小鼠的破骨细胞(osteoclast,OC),通过检测TRAP阳性细胞数及骨吸收陷窝面积,比较鸭胚与小鼠的OC形态、数目及活性。结果表明,鸭胚OC形状不规则、体积较大、核多且主要分布于胞质,而小鼠OC形状较规则、体积小、核多且大多分布于细胞边缘;随机选取视野中,鸭胚与小鼠OC数差异不显著;而鸭胚OC骨吸收陷窝面积显著大于小鼠OC骨吸收陷窝面积(P<0.05)。表明鸭胚与小鼠OC均具有OC的普遍特征,但两者在骨吸收活性方面存在差异。
Osteoclasts were isolated from duck embryo and mice respectively,and the morphology number and activity of the two sources of osteoclasts were compared by tartrate resistant acid phosphatase(TRAP)staining and bone resorption pits assaying.The results showed that morphology of duck embryo osteoclasts was irregular,large volume,multinucleated,and most of the nuclei were located in cytoplasm.While the morphology of mice osteoclasts was regular relatively,small volume,multinucleated and most of the nuclei were located in the margin of cells.The number of duck embryo and mice osteoclasts in random visual fields had no significant difference.However,the area of duck embryo osteoclasts bone resorption pits was significantly greater(P<0.05)than that of mice osteoclasts.These results demonstrated that the two sources of osteoclasts all possessed common characteristics of osteoclast,but their bone resorption activity was different.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2015年第6期953-956,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31172373
31372495
31302154)
江苏省高校优势学科建设工程资助项目
江苏省动物重要疫病与人兽共患病防控协同创新中心资助项目(2013)
教育部博士点基金资助项目(20113250110003)
2015年度安徽省自然科学基金青年资助项目(1508085QH/72)
关键词
鸭胚
小鼠
破骨细胞
特性
duck embryo
mice
osteoclast
characteristic
