摘要
目的:研究甲基泼尼松龙对大鼠尺骨皮质骨骨量、微结构及微损伤的影响。方法:20只3.5月龄雌性Sprague-Dawley大鼠随机分为甲基泼尼松龙[3.5 mg/(kg·d)]干预组和对照组,每组10只。干预后9周处死,处死前测量体质量及全身骨密度。右侧前肢经肱骨肩关节离断后,行尺骨单轴疲劳,疲劳后取尺骨中段行大块组织碱性品红染色包埋,并行骨组织形态计量学测定及微损伤定量分析。结果:甲基泼尼松龙干预组大鼠体质量、全身骨密度和左侧尺骨骨密度分别较对照组下降15%,6.4%和4.3%(均P<0.05),尺骨内径周长和骨髓腔面积较对照组分别增加23.3%和32%(均P<0.05),外径周长较对照组下降3.1%(P>0.05);尺骨皮质骨骨面积和截面总面积与对照组相比较,差异无统计学意义(均P>0.05)。干预组大鼠的尺骨微损伤数量、微损伤数量密度、损伤表面长度密度较对照组分别增加43%,48%和50%(均P<0.05),微损伤平均长度无明显改变(P>0.05)。结论:甲基泼尼松龙能显著诱导大鼠尺骨皮质骨骨量丢失、微结构及微损伤退变。
Objective: To explore the effect of methylprednisolone on bone mass, microarchitecture and microdamage in cortical bone of ulna in rats.Methods: Twenty female Sprague-Dawley rats(3.5 months old) were randomly assigned to two groups: a treatment group and a control group(n=10 per group). The treatment group was subcutaneously injected with methylprednisolone 3.5 mg/(kg.d) while the control group was subcutaneously injected with same volume of vehicle(saline). Rats were sacrificed at 9 weeks after the treatments. Before the sacrifice, the body weight and total bone mineral density(BMD) were measured. The right forelimb was separated through humeral shoulder and then single axial fatigue loading was performed on the right ulna. After fatigue load, the middle ulna section was bulkstained in basic fuchsin. Bone histomorphometry and microdamage analysis were performed on the middle ulna section.Results: Compared with the control group, the body weight, total bone BMD and ulnas BMD in the treatment group were decreased by 15%, 6.4% and 4.3% respectively(all P<0.05); the ulna inner perimeter and marrow area in the treatment group were increased by 23.3% and 32%, respectively(both P<0.05), while the outer perimeter were decreased by 3.1%(P>0.05). There was no significant difference in the cortical and total area between the 2 groups(both P>0.05). The number of microcrack, microcrack density and microcrack surface density in the treatment group were increased by 43%, 48% and 50%, respectively, compared with those in the control group(all P<0.05), but there was no significant difference in the mean length of microcrack between the 2 groups(P>0.05).Conclusion: Methylprednisolone can significantly induce the bone loss and the deterioration of microarchitecture and microdamage in ulna of rats.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2015年第1期25-30,共6页
Journal of Central South University ：Medical Science
关键词
糖皮质激素
骨密度
微结构
微损伤
glucocorticoid
bone mineral density
miroarchitecture
microdamage
