摘要
旨在寻找影响京海黄鸡新城疫和传染性支气管炎抗病性状的SNP位点。本研究采用简化基因组测序的方法,检测京海黄鸡基因组的SNP位点,并对这些位点与新城疫和传染性支气管炎性状进行关联分析。结果表明:在基因组水平上有1个与京海黄鸡新城疫抗病性状显著相关的SNP位点,7个与该性状潜在显著的SNPs位点,并将这些位点定位到5个基因上,分别为EEA1、CARS2、SCML2、GRP20以及TOMIL2基因,这些基因均可能影响或调控机体的抗病及免疫能力,可以考虑作为京海黄鸡新城疫抗病性状的候选基因作为后续研究。没有发现与京海黄鸡传染性支气管炎显著相关的SNP位点,该性状可能是复杂性状,受到多种因素的影响。因此,本研究发现的几个标记位点可能对京海黄鸡的新城疫抗病性状存在一定的影响,可以作为候选基因,为京海黄鸡抗病育种过程中的标记辅助选择提供参考资料。
The objective of this study was to seek SNPs that influence Jinghai Yellow chicken's ND and IB disease resistance character.The SNPs in Jinghai Yellow chicken were detected by SLAF-seq method and then the association of SNP sites with ND and IB disease resistance character were analyzed.The result showed that one SNP was significantly associated with ND disease resistance character,7SNPs were potential significantly associated with ND disease resistance,and they were located in 5genes:EEA1,CARS2,SCML2,GRP20 and TOMIL2;All of the 5genes might influence or regulate organism's disease resistance and immune ability and could be the candidate genes for Jinghai Yellow chicken's disease resistance character.No SNP was found that was significantly associated with IB disease resistance character,this trait was complex and was rugulated by many factors.The results showed the 5genes had influence on Jinghai Yellowchicken's ND disease resistance characeter,and could provide the reference data for marker assisted selection of Jinghai Yellow chicken's disease resistant breeding process.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2015年第2期196-203,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省高校自然科学基金(12KJB230003)
国家自然科学基金(31201793)
国家肉鸡产业技术体系(nycytx-42-G1-05)
江苏高校优势学科建设工程
江苏省动物遗传繁育与分子设计重点实验室
