摘要
目的探讨亲环素A(Cy PA)/CD147在兔动脉粥样硬化易损斑块中的表达。方法新西兰雄性白兔16只,3月龄,随机分为对照组和模型组,每组8只。利用液氮损伤内膜+高脂饮食的方法建立易损斑块模型,对照组正常饮食,不做任何处理,0周和13周分别检测兔血清C反应蛋白(CRP)、Cy PA和CD147表达水平。术后13周处死动物,取血管行HE染色、Masson染色,观察血管形态,并做巨噬细胞、Cy PA、CD147免疫组织化学染色。结果对照组未见斑块形成,模型组兔颈动脉形成典型的易损斑块,斑块内Cy PA和CD147表达丰富,呈带状分布特征,两者在同一斑块中具有共区域性;13周时模型组兔血CRP、Cy PA和CD147水平较0周及同时期对照组均明显升高(P<0.05)。结论易损斑块中Cy PA、CD147表达丰富,Cy PA和CD147是易损斑块的危险因素,其表达与易损斑块的存在呈正相关。
Aim To explore the expressions of cyclophilin A( Cy PA) /CD147 in rabbit atherosclerotic vulnerable plaques. Methods 16 healthy male New Zealand rabbits( 3 months) were randomly divided into control group and model group,8 rabbits in each group. Rabbit model with typical atherosclerotic vulnerable plaque was developed by injuring carotid intima with liquid nitrogen combined with high-fat diet. Meanwhile,the control group was fed with standard diet,and without injuriy. Expressions of C-reactive protein( CRP),Cy PA and CD147 in serum of rabbits were tested at the beginning and the 13 th week. All rabbits were sacrificed after 13 weeks. Then paraffin-embedded sections were used for HE staining and Masson trichrome staining to analyze the changes of vascular morphology. Additionally,macrophagocyte,Cy PA and CD147 monoclonal antibodies were applied for immunohistochemistry staining. Results Atherosclerotic vulnerable plaques were found in model group but not in control group. There were rich expressions of Cy PA and CD147 with zonal distribution and co-location in the same atherosclerotic vulnerable plaques. The expressions of Cy PA,CD147 and CRP in serum increased significantly in model group compared with those in control group at the 13 th week( P < 0. 05). Conclusions High expressions of Cy PA and CD147 were found in atherosclerotic vulnerable plaques,and the expressions of Cy PA and CD147 were the risk factors of the atherosclerotic vulnerable plaques,which have a positive correlation with the existence of atherosclerotic vulnerable plaques.
出处
《中国动脉硬化杂志》
CAS
北大核心
2015年第5期495-499,共5页
Chinese Journal of Arteriosclerosis
