摘要
为了补充现有苜蓿(Medicago sativa)种质资源的遗传多样性信息,采用SSR分子标记技术对1份野生紫花苜蓿种质和41份栽培紫花苜蓿品种的遗传多样性进行了研究。15对引物在供试苜蓿材料中共获得231条扩增带,其中163条具有多态性。引物的多态位点百分率、Nei’s基因多样性指数和Shannon信息指数的平均值分别为71.55%、0.210 0和0.326 3。供试材料间的相似系数介于0.641~0.913,平均为0.791,栽培品种间的平均相似系数相对较大,野生种质资源与栽培品种间的平均相似系数相对较小。聚类分析表明,供试材料在相似系数0.778处可聚为五大类,单独聚为类的陇东野生紫花苜蓿、CW 200和CW 787都表现出了与其他材料间较远的亲缘关系。基于主成分分析图,可把供试材料分为四大类,其中第Ⅰ、Ⅱ类与第Ⅲ、Ⅳ类材料间的亲缘关系较远。综上,供试苜蓿种质资源具有较丰富的遗传多样性,部分种质资源表现出了相对独立的遗传特性。
In order to reveal the genetic diversity of alfalfa germplasm resources,the genetic diversity of one wild and 41 cultivated alfalfa accessions were analyzed using SSR markers.The results showed that there were totally231 alleles and 163 polymorphic alleles generated from these 42 alfalfa accessions by 15 SSR primers.The mean values of polymorphic percentage,Nei's gene diversity and shannon's information index for these 15 primer pairs were 71.55%,0.210 0 and 0.326 3,respectively.The similarity coefficient of accessions ranged from 0.641 to0.913 with an average of 0.791,and the mean value of similarity coefficient between wild alfalfa accessions and cultivars was lower than that among cultivars.Cluster analysis classified accessions into five defined groups at the genetic similarity value of 0.778,among which Longdong wild alfalfa,CW 200 and CW 787 were clustered into three independent groups,indicating that these accessions had relatively distant genetic relationship with the others.According to principal component analysis,the accessions can be divided into four groups,and the accessions in the Ⅰ and the Ⅱ groups had relatively distant genetic relationship with those in the Ⅲ and the Ⅳ groups.In conclusion,the alfalfa accessions used in the present study had relatively high genetic diversity,and some of which had relatively independent genetic characteristics.
出处
《草业科学》
CAS
CSCD
北大核心
2015年第3期372-381,共10页
Pratacultural Science
基金
新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201103)
新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201405)
农业部全国畜牧总站"牧草种质资源保种"项目
关键词
苜蓿
遗传多样性
亲缘关系
SSR
alfalfa
genetic diversity
genetic relationship
SSR
