摘要
生长素酰胺水解酶(IAA-Leucine Resistant1-like Hydrolase,ILL)可催化生长素结合态释放游离态生长素(IAA)。为了深入研究桃ILL基因家族的功能,对桃基因组中ILL基因家族成员的数量、在染色体骨架的分布、编码蛋白质的结构、基因表达模式和编码氨基酸的进化树分类等方面进行了研究。桃基因组中共鉴定出了9个ILL基因,它们分布在4个染色体骨架上(scaffold 1、2、3和7)。在桃中编码ILL蛋白家族的基因成员分别为:Pp ILR1、Pp ILR2、Pp ILR3、Pp ILR4、Pp ILL1、Pp ILL2、Pp ILL3、Pp ILL4和Pp ILL5,所有这些预测的蛋白都含有M20结构域和M20二聚化结构域。运用荧光定量实时PCR技术对该基因家族在桃果实生长发育各阶段的表达水平进行了分析,结果显示:ILL基因家族中的数个成员在果实不同发育阶段表达水平有变化,其中Pp ILR1的表达量在果实生理成熟期明显上调。
The IAA-Leucine Resistant1-like Hydrolase(ILL)genes is thought to regulate the release of free indole-3-acetic-acid(IAA)which is involved in the regulation of fruit development and others developmental processes. In order to further study the function of ILL family genes in peach,the member number of ILL gene family was confirmed;Thereafter their distribution on the scaffold,putative proteins,phylogenetic classification,and expression patterns were analyzed. We identified 9 ILL genes in the entire peach genome,which distributed across 4 scaffolds(1,2,3 and 7). The members of the ILR1-like family genes were Pp ILR1,Pp ILR2,Pp ILR3,Pp ILR4,Pp ILL1,Pp ILL2,Pp ILL3,Pp ILL4 and Pp ILL5. All of their putative proteins contained M20 and M20 dimerisation domains. The relative expression levels in mesocarps of peach fruit at different developmental stages were also detected by quantitative real-time PCR. The results showed that several genes exhibited different expression patterns during fruit development. Moreover,the transcript level of Pp ILR1 was significantly up-regulated at the late-ripening stage.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第2期243-251,共9页
Acta Horticulturae Sinica
基金
国家‘863’计划项目(2011AA10020606)
国家科技支撑计划项目(2013BAD02B03-2)
关键词
桃
果实发育
生长素酰胺水解酶
生长素
溶质型
Prunus persica
fruit development
IAA-Leucine Resistant1-like Hydrolase
indole-3-acetic-acid
melting-flesh
