摘要
对反义MLPK基因在甘蓝柱头特异启动子SLR的驱动下,通过农杆菌介导转化法将其导入高度自交不亲和甘蓝材料‘TF’。转基因甘蓝T0代植株定量PCR分析结果显示,不同的转MLPK反义基因单株内源MLPK m RNA积累量具有明显差异,其中转基因植株花期柱头内源MLPK m RNA积累量明显低于野生型对照。花粉原位萌发的荧光显微镜观察结果显示,转基因甘蓝植株花期自交后,吸附在柱头上的花粉粒大量萌发,且穿过柱头的花粉管明显增加,并导致花期自交结籽数上升,转基因植株花期和蕾期自交亲和指数均明显高于野生型对照植株。结果表明,下调MLPK基因表达能部分打破甘蓝自交不亲和,提高其花期自交结籽能力。
An antisense MLPK gene controlled by a stigma-specific promoter SLR of Brassica oleracea was introduced into a self-incompatibility B. oleracea‘TF'through Agrobacterium mediated transformation. Real-time PCR expression analysis of the MLPK gene in T0 generation transgenic cabbage plants was performed,significant different transcription levels of the MLPK gene in transgenic plants were observed,and the transcription level of MLPK in open flower stigmas of the transgenic plants were greatly reduced compared with the wild type plants. In situ fluorescence microscopy results showed a large number of pollen germination on the stigmas of transgenic plants,and markedly increased pollen tubes penetrated the stigma after self-pollination of open flower,which leads to significantly increased numbers of seed. Moreover,both the flowering and bud stage compatibility index of transgenic plants were significantly higher than the wild type. The results demonstrated that down-regulation expression of the MLPK gene in stigma can result in a partial breakdown of B. oleracea self-incompatibility,and promote seed setting after self-pollination during flowering stage.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第2期252-262,共11页
Acta Horticulturae Sinica
基金
中央高校基本科研业务费项目(XDJK2009B024)
国家科技支撑计划项目(2012BAD02B01-4)
重庆市科技攻关项目[CSTC2012GG(8005)]
重庆市自然科学基金项目(cstc2011jj A80014)
关键词
甘蓝
自交不亲和性
反义MLPK基因
Brassica oleracea var.capitata
self-incompatibility
antisense MLPK gene
