摘要
以‘中油桃9号’(白肉)及其黄肉芽变的果肉为试材,采用HPLC法对类胡萝卜素的积累水平进行定性和定量分析,实时荧光定量PCR法对类胡萝卜素代谢关键基因的表达水平进行分析。结果表明:在幼果期,‘中油桃9号’与突变体的果肉颜色无明显差异,但在果实成熟时差别很大;幼果期时‘中油桃9号’与突变体的类胡萝卜素总量相近,以β–胡萝卜素、紫黄质和叶黄质为主;果实成熟期‘中油桃9号’的类胡萝卜素总量比突变体高很多,呈现出高含量的紫黄质、β–胡萝卜素和玉米黄质;实时定量表达分析表明,果实成熟期‘中油桃9号’的CCD4转录水平比突变体高得多。果实成熟时CCD4基因的表达差异可能是导致‘中油桃9号’与突变体类胡萝卜素积累差异的主要原因。
Two nectarine cultivars,‘CN9'(white-fleshed)and its yellow-fleshed bud sport mutant ‘CN9Y',were taken as materials to investigated carotenoids metabolism and genes expression. Qualitative and quantitative levels of carotenoids were analzed by HPLC,the expression levels of carotenoid biosynthetic genes were detected by real time q PCR method. The results included that no distinct difference in flesh color appeared between‘CN9'and its mutant during early development stage,while at the ripening stage,differences in flesh color became dramatic. At early development stage,the total carotenoid levels were similar in‘CN9'and‘CN9Y'fruits,dominated by the presence of β-carotene,violaxanthin,and lutein. The total carotenoid content was much higher in‘CN9Y'than in‘CN9'at the ripening stage,exhibiting higher quantities of violaxanthin,β-carotene,and zeaxanthin. Real-time quantitative expression analysis showed that the CCD4 transcript levels were dramatically higher at late ripening stages in‘CN9'with respect to‘CN9Y'. These results indicated that the difference in the expression level of CCD4 between‘CN9'and its yellow-fleshed mutant is likely to be the major determinant of carotenoid accumulation levels.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第4期623-632,共10页
Acta Horticulturae Sinica
基金
国家‘863’计划项目(2011AA10020606)
国家自然科学基金项目(30871682)
关键词
油桃
白肉
黄肉
芽变
类胡萝卜素
基因表达
nectarine
white-flesh
yellow-flesh
bud sport mutant
carotenoids
gene expression
