摘要
目的 探讨c kit蛋白和mRNA在慢传输性便秘结肠的表达。方法 乙状结肠标本取自 12例慢传输性便秘患者和 8例年龄相匹配的非梗阻性结直肠癌患者。用Westernblot方法检测c kit蛋白表达 ,用RT PCR方法检测mRNA表达情况。结果 聚丙烯凝胶电泳结果显示c kit蛋白质一 14 5× 10 3 的条带 ,与对照组相比 ,慢传输性便秘结肠c kit蛋白表达明显下降 (P =0 .0 2 1) ;RT PCR产物经琼脂糖凝胶电泳显示c kit基因和 β actin(内参照 )两条带 ,经半定量分析发现c kitmRNA表达降低 (P =0 .0 2 9)。结论 慢传输性便秘乙状结肠c kit蛋白和mRNA表达均明显降低 ,提示c
Objective To examine the expressions of c kit mRNA and c kit protein in the colon of patients with slow transit constipation (STC). Methods The sigmoid colon samples from 12 STC patients and 8 age matched patients with nonobstructed colorectal cancer were used in this study. Expression of c kit protein was detected by Western blot analysis, and expression of c kit mRNA was detected by RT PCR. Results Compared with those of controls, the c kit mRNA and protein expressions were significantly declined in the colon of slow transit constipation ( P <0.05). Conclusion Significant downregulation of c kit gene and protein expressions may play an important role in the decreases in interstitial cells of Cajal in slow transit constipation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第12期1052-1054,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 30 30 0 156 )
重庆市科技基金资助项目 ( 2 0 0 2 74 38)~~
