摘要
基于4 (2 吡啶偶氮) 间苯二酚(PAR)在碱性条件下催化铁氰化钾 鲁米诺体系产生很强的化学发光的原理,对影响PAR发光的条件进行了优化选择,得出该体系下PAR的相对标准偏差为2.7%,线性范围为1.0×10-5～1.0×10-7mol·L-1;检出限为5.7×10-8mol·L-1。利用PAR对许多金属离子的络合性,得出测定某些金属离子的检出限。并同时建立了测定PAR的化学发光新方法。
In an alkaline medium of pH 12.7, the chemiluminescent reaction between potassium ferricyanide and luminol was catalyzed by the presence of microamounts of PAR leading to an enhancement of the CL-intensity. The parameters of this CL reaction were studied and optimized. Under the optimum condition a linear relationship was found between the relative CL intensity (I_R) and the concentration of PAR in the range of 1.0×10^(-5)～1.0×10^(-7)mol·L^(-1), with a detection limit of 5.7×10^(-8)mol·L^(-1) of PAR. Precision test at the concentration level of 1.0×10^(-7)mol·L^(-1) of PAR was made and RSD′s (n=6) found was 2.7%. Based on the color reaction of PAR with many metal ions, CL methods for the determination of the respective metal ion can be established by measuring the decrease of CL-intensity, due to the formation of M^(n+)-PAR chelate. The CL determination of several metal ions (including Co^(2+), Ni^(2+), Pb^(2+), Mo(Ⅵ), Zn^(2+), Cd^(2+) and In^(3+)) were studied with their detection limits given. The selectivity of the determination of metal ions can be remarkably improved, if separation by chromatography or by capillary electrophoresis is applied. A disign of FI system for the CL-reaction was also described.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2004年第7期375-377,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
