摘要
目的 :探讨细胞外调节蛋白激酶 (ERK)与阿尔茨海默病 (AD)发病机制的关系。方法 :应用穹窿海马伞切断AD大鼠模型 ,分别用同位素方法和蛋白质印迹检测ERK的水平。结果 :同位素方法检测穹窿海马伞切断AD大鼠海马ERK的活性 ,发现手术组手术侧为 (13.4 6± 3.2 3) pmol·mg-1·min-1,明显高于正常对照组 (10 .80± 1.5 0 )pmol·mg-1·min-1、假手术组 (10 .74± 1.6 2 )pmol·mg-1·min-1和手术组非手术侧 (10 .79± 2 .2 3)pmol·mg-1·min-1;Westernblot显示ERK水平在手术组高于正常对照组。结论 :穹窿海马伞切断AD大鼠海马ERK升高 ,说明ERK信号转导途径在AD发病早期有改变。
Objective: To study the relationship of extracellular regulated protein kinase(ERK) and mechanism of Alzheimer's disease( AD).Methods:Rat model of AD was made by transection of rat fimbria-fornix. The activity of ERK was determined by using isotope and Western blot.Results:Activity of ERK tested by using isotope in operating side of operated group was(13.46±3.23) pmol·mg -1 ·min -1 ,which was significantly higher than(10.80±1.50) pmol·mg -1 ·min -1 of normal group,(10.74±1.62)pmol·mg -1 ·min -1 of pseudo-operated group,and (10.79±2.23)pmol·mg -1 ·min -1 in unoperating side of operated group. By using Western blot method,level of ERK in operated group was higher than that of normal group.Conclusion:The level of ERK increased in hippocampus of rat model of AD,which showed changes of signal transduction route in the early phase.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2004年第4期302-303,311,共3页
Journal of China Medical University
关键词
阿尔茨海默病
穹窿海马伞切断
大鼠
细胞外调节蛋白激酶
Alzheimer's disease
transection of rat fimbria-fornix
rats
extracellular regulated protein kinase
