摘要
作者应用抗不同抗原决定簇的Ⅲ型胶原单克隆抗体CAb-15,CAb 16,分别对正常肝、慢性肝炎、肝硬变和肝细胞癌的福尔马林固定、石蜡包埋组织进行免疫组化定位.结果在慢性迁延性肝炎-慢性活动性肝炎肝硬变之间,Ⅲ型胶原呈递增趋势,在慢性活动性肝炎,肝硬变及肝细胞癌组织中部分细胞与抗x_1[Ⅲ]链的胶原抗体呈阳性反应,表明肝细胞及肝细胞癌细胞具有合成Ⅲ型胶原的能力,在肝纤维化和癌间质形成过程中具有重要作用,同时也提示Ⅲ型胶原在细胞内、外的存在状态有所区别.
Immunohistochemical localization of type Ⅲcollagen was performed in sections of hepatitis, cirrhosis and hepatocellular carcinoma by ABC method with monoclonal antibodies (CAb 15, CAb 16) which reacted with x1 (Ⅲ) polypeptide chains and triple helical domains respectively. It was shown that positive staining of chronic active hepatitis and cirrhosis was stronger and more diffuse than that of chronic persistent hepatitis; some hepatocytes of chronic active hepatitis, cirrhosis and hepatocellular carcinoma cells showed positive CAb 15, and negative CAb 16-This result suggested that hepatoeytes and cancer cells were able to synthesize type Ⅲcollagen, it played a significant role in formation of fibrosis and tumor matrix. It also suggested that extracellular and intracellular collagen may be different in structure.
出处
《第四军医大学学报》
1993年第1期22-25,共4页
Journal of the Fourth Military Medical University
关键词
胶原
Ⅲ型
肝炎
肝硬变
肝肿瘤
collagen,type Ⅲ
hepatitis
liver cirrhosis
hepatocellular carcinoma
mmunohislochemistry
