摘要
目的:建立以高相液相色谱法测定药用植物油——薏苡仁油中的脂肪酸的方法。方法:以2,4’-二溴苯乙酮为衍生化试剂,18-冠-6醚为相转移催化剂,采用HiQ sil C8(250 mm×4 mm,5μm)反相柱,波长254 nm。以乙腈-水(85:15)为流动相等度洗脱,柱温30℃,设定流速为1.5 mL·min-1。正十七烷酸为内标,一次基线分离5种脂肪酸。结果:亚油酸的线性范围为0.026-0.387μg,软脂酸的线性范围为0.014-0.207μg,油酸线性范围为0.027-0.404μg,硬脂酸的线性范围为0.011-0.160μg,相关系数均为0.9999。平均回收率分别为100.4%,102.2%,99.4%,104.5%,RSD分别为1.0%,1.9%,0.7%,2.4%。结论:本方法重现性好,定量准确,可作为薏苡仁油中脂肪酸测定的定量方法。
Objective:A method was developed for the determination of fatty acid in Oleum Coicis by pre - column derivation HPLC. Method:Fatty acids were derivatized with p -bromophenacylbromide as derivative and 18 -crown -6 as catalyst. The method used C8 HiQ sil C8(250 mm×4 mm,5 μm)column material and isocratic acetonitrile -water eluent and the internal standard was heptadecanoic acid. The detection wavelenghth is 254 nm. Column temperature is fixed at 30℃, and the flow rate is 1. 5 mL·min -1. Results: The standard curves of linoleic, palmitic,oleic,steraric acid are linear within the range of 0. 026 -0.387μg,0.014 -0.207μg,0.027 -0.404μg, 0.011 -0.160uuuuuug and the coefficient are 0.9999,0.9999,0.9999,0.9999. The four fatty acid recoveries are 100.4% ,102. 2% ,99.4% ,104. 5% and the RSD are 1. 0% ,1. 9% ,0. 7% ,2. 4% individually. Conclusion:Five fatty acid were separated within 30 minutes during a single run. The present method is reliable and relatively simple for the determination of fatty acid in Oleum Coicis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第3期249-252,共4页
Chinese Journal of Pharmaceutical Analysis
基金
辽宁省重大课题项目(编号:2002226005)
