摘要
目的 建立流动注射抑制化学发光测定乙酰螺旋霉素的新方法。方法 在碱性介质中乙酰螺旋霉素能强烈抑制luminol K3Fe(CN) 6 化学发光反应。本文以乙酰螺旋霉素在一定浓度范围内与luminol K3Fe(CN) 6 化学发光强度降低值的呈线性关系为基础 ,结合流动注射技术 ,快速测定乙酰螺旋霉素。结果 测定的线性范围为 0 1～ 10 0μg·L- 1 ,检测限为 4 0ng·L- 1 ( 3σ) ,RSD小于 3 0 % (n =8)。结论 该法简便快速、灵敏度高、选择性好 ,可用于药物、人血清中的乙酰螺旋霉素含量的测定 。
Aim To establish a new and simple flow injection method for the rapid determination of acetylspiramycin (ASPM). Methods ASPM was determined by chemiluminescence (CL) method combined with flow injection (FI) technology, which was based on the inhibitive effect of ASPM on the chemiluminescence reaction of the luminol K 3Fe(CN) 6 system. Results The decrease of chemiluminescence intensity was proportional to the logarithm of ASPM concentration (0 1-100) μg·L -1 , the detection limit was 40 ng·L -1 (3σ). The whole process, including sampling and washing, could be completed in 0 5 min with a RSD less than 3 0% ( n =5). Conclusion The FI CL method is of both high sensitivity and good selectivity giving a throughput of 120 h -1 . The proposed method was applied successfully to the determination of ASPM in pharmaceutical preparations and human urine without any pre treatment. It was found that the ASPM concentration reached its maximum after being orally administrated for two hours.
出处
《药学学报》
CAS
CSCD
北大核心
2004年第1期64-67,共4页
Acta Pharmaceutica Sinica
基金
NaturalScienceFoundationofEducationofShaanxiProvince(0 1JK0 71)
