摘要
目的 :研究心房颤动患者心房肌乙酰胆碱敏感钾通道基因表达的改变 ,揭示人心房颤动时心房有效不应期缩短和有效不应期频率适应性下降的机制。方法 :选择 2 7例行开胸手术的病人 ,获取右心耳部位手术附属物 5 0mg～ 10 0mg ,采用一步法逆转录聚合酶链反应技术 ,测定心房组织中乙酰胆碱敏感钾通道mRNA的变化。结果 :房颤患者心房肌乙酰胆碱敏感钾通道mRNA水平较窦性患者明显降低 (目的基因与GAPDH基因之比 :窦性组0 .6 0 0 4± 0 .2 0 0 0 (n =11) ,房颤组 0 .3170± 0 .196 3(n =16 ,P <0 .0 5 )。结论 :房颤患者心房肌的乙酰胆碱敏感钾通道mRNA表达水平下调 ,表明乙酰胆碱敏感钾通道可能参与了房颤的电重构 ,有助于房颤的发生及维持。
Objective: Alterations in gene expression of acetylcholine-activated potassium channel in patients with atrial fibrillation were studied to reveal the mechanism underlying the shortening of the atrial effective refractory period and the loss of rate adaptive shortening of the atrial effective refractory period in human atrial fibrillation. Methods: 50～100mg tissues from right atrial appendages were obtained from 27 patients who were undergoing open heart surgery. The mRNA levels of acetylcholine-activated potassium channel were measured by one-step reverse transcription polymerase chain reaction. Result: It is found that the mRNA levels of Kir3.4 were significantly reduced in patients with atrial fibrillation(gene of interest/GAPDH ratios :0.6004±0.2000 in SR group(n=11),0.3178±0.1963 in AF group(n=16,P<0.05). Conclusions: Expression of gene of acetylcholine-activated potassium channel is downregulated in atrial fibrillation, which suggests that acetylcholine-activated potassium channel possibly participates in the electric remodeling of atrial fibrillation and contributes to the occurring and maintenance of atrial fibrillation.
出处
《泸州医学院学报》
2004年第3期192-195,共4页
Journal of Luzhou Medical College
基金
教育部重点科研项目 (资助号 :0 310 9)
关键词
心房颤动
乙酰胆碱敏感钾通道
逆转录聚合酶链反应
Atrial fibrillation(AF)
Acetylcholine-activated potassium channel(KACh )
Reverse transcription polymerase chain reaction(RT-PCR)
