摘要
目的 探讨32 P、大蒜素 (DT)及两者联合包被支架对犬冠状动脉再狭窄的预防作用。方法 制作蛋白质包被的32 P胶体 (n =6 )、DT(n =5 )及32 P +DT联合包被的蛋白质涂层支架 (n=6 )。 10只杂种犬 ,分别置入以上 3种支架 ,同时置入单纯蛋白质涂层支架作为对照组 (n =5 )。术后 6个月重复冠状动脉造影后 ,处死动物 ,取材 ,进行形态学观察和组织图像分析。结果 组织学观察发现 ,3种药物包被支架组的内膜面积 [32 P :(2 6 3± 0 4 8)mm2 ,DT :(2 5 0± 0 4 9)mm2 ,32 P +DT :(1 4 2± 0 4 1)mm2 ]小于对照组 [(4 87± 0 80 )mm2 ,P =0 0 0 1],联合包被组的内膜面积小于各自的单一包被支架组(P≤ 0 0 0 5 ) ;3种药物组支架置入段的血管面积狭窄率 [32 P :(32 4 8± 4 79) % ,DT :(30 0 4±5 4 3) % ,32 P +DT :(17 2 1± 4 0 5 ) % ]显著低于对照组 [(5 4 6 2± 6 82 ) % ,P <0 0 0 1],联合包被组面积狭窄率显著低于单一包被支架组 (P≤ 0 0 0 1)。血管造影示 ,3种药物包被组直径再狭窄率低于对照组 (P <0 0 0 1) ,联合包被支架组直径再狭窄率低于单一包被支架组 (P≤ 0 0 0 5 )。未发现边缘效应。结论 32 P和DT联合包被支架可预防内膜过度增生 ,其疗效优于单纯32 P或DT包被支架。
Objective To asesses the efficiency of 32 P and diallyl trisulfide (DT)-coated coronary stents on neointimal proliferation in a canine model of restenosis. Methods 32 P (740 kBq/stent), DT (210 μg/stent) alone and jointly coated stents were deployed with mild oversizing in both the left circumflex coronary artery (LCX) and the left anterior descending coronary artery (LAD) of 10 dogs. The implantation of protein-coated stents was used as the control. The therapeutic effect was assessed 6 months after the intervention. The dogs were sacrificed and the vessels were excised. For morphologic examinations the tissue sections were stained with HE and Van Gieson. Results Compared with the control stents, the neointimal area reduced in stents coated with 32 P, DT alone and 32 P+DT [ 32 P:(2.63±0.48)mm 2, DT:(2.50±0.49)mm 2, 32 P+DT:(1.42±0.41)mm 2 vs control group:(4.87±0.80)mm 2, P=0.001]. 32 P+DT was more effective than either of them alone for reducing the neointimal area (P≤0.005). Both jointly and alone coated stents of 32 P, DT decreased the percent area stenosis [ 32 P:(32.48±4.79 )%, DT: (30.04±5.43)%, 32 P+DT: (17.21±4.05)% vs control group:(54.62±6.82)%, P<0.001], whereas the percent area stenosis was significantly smaller in 32 P+ DT group compared with 32 P or DT-coated stents group (P≤0.001). Angiographic restenosis (diameter stenosis) was lighter in stents coated with 32 P, DT and 32 P+DT compared with control stents (P<0.001). 32 P+DT was more effective than 32 P or DT alone for reducing diameter stenosis (P≤0.005). No evidence of ‘edge effect’ was seen in adjacent, nonstented coronary segments. Conclusion Coronary stents coated with 32 P plus DT produced a significantly reduced neointimal hyperplasia and restenosis rate in the canine LCX and LAD 6 months after stents implantation.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
2004年第3期161-163,i002,共4页
Chinese Journal of Nuclear Medicine
基金
湖北省九五攻关项目 (962p1 1 0 1 )
