摘要
目的 探讨体外受精过程中胚胎污染的发生率、污染菌种和污染的来源。方法 回顾性分析 1999年 1月至 2 0 0 3年 6月 ,2 174个体外受精 胚胎移植 (IVF ET)周期中胚胎污染的发生率及污染菌种 ;随机抽取IVF ET中 6 1例精液分析正常男性的精液和 34例卵泡液进行细菌培养。结果发生胚胎污染 11例 ,发生率为 0 5 1% (11/ 2 174 ) ;污染菌种主要为大肠埃希菌 (4例 )和真菌 (4例 )。精液原液、处理后的精液上清液、精液原液与培养液的混合液及卵泡液细菌培养的阳性率分别为97% (2 9/ 30 )、10 % (3/ 30 )、6 % (2 / 31)及 9% (3/ 34)。结论 在体外受精的过程中 ,可发生部分胚胎污染 ,污染的菌种主要为大肠埃希菌和真菌。精液中的细菌可能是胚胎污染的主要来源。
Objective To investigate the incidence of microorganism contamination in in vitro fertilization-embryo transfer (IVF-ET) and to determine the sources of microorganism. Methods Two thousand one hundred and seventy-four cycles of in vitro fertilization from January 1999 to June 2003 were evaluated retrospectively and bacterial cultures were performed in 61 semen samples from asymptomatic men with normal semen parameters and in 34 follicle fluid samples from infertility women through oocyte picking up procedures. Results Microorganisms were found in 11 cases. The incidence of their contamination in IVF culture system was 0.51% and the most common microorganisms were Escherichia coli and fungi. Microorganisms were detected in 97% of unprocessed semen, 10% in processed semen, 6% in semen mixed with media and 9% in follicle fluid. Conclusions The incidence of microorganism contamination was 0.51% and the most common microorganisms were Escherichia coli and fungi. Semen may have the potential to contaminate IVF culture system.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第6期382-384,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
体外受精
胚胎污染
细菌
精液
Fertilization,in vitro
Embryo transfer
Bacteria
Semen
