摘要
目的 探讨人乳头状瘤病毒 (HPV)负荷量与子宫颈癌及其癌前病变的关系。方法 2 0 0 1年 12月～ 2 0 0 2年 5月 ,对山西省宫颈癌高发现场的 90 75例 35～ 5 0岁的已婚妇女 ,采用杂交捕获二代(HC Ⅱ )方法定量检测宫颈HPV DNA的含量 (以此表示宫颈HPV负荷量 )。对其中HPV DNA阳性妇女 ,随后进行阴道镜检查、宫颈多点活检和颈管诊刮术。结果 2 0 87例HPV阳性妇女中 ,子宫颈炎14 0 2例 (6 7 2 % ) ,子宫颈癌前病变 [包括子宫颈上皮内瘤变 (CIN)Ⅰ～Ⅲ ]6 6 3例 (31 8% ) ,子宫颈浸润癌 (SCC) 2 2例 (1 1% )。急、慢性宫颈炎的HPV DNA含量分别为 10 8± 13和 15 0± 11,两者比较 ,差异无显著性 (P =0 2 5 5 ) ;不同级别子宫颈癌前病变的HPV DNA含量 ,CINⅠ为 332± 2 9,CINⅡ为 35 8±35 ,CINⅢ为 370± 31,均明显高于宫颈炎 (P =0 0 0 0 ) ,但不同级别CIN之间比较 ,差异无显著性 (P >0 0 5 ) ;SCC的HPV DNA含量为 5 93± 86 ,显著高于不同级别CIN和宫颈炎的HPV DNA含量 (P <0 0 5 ,P <0 0 1)。结论 HC Ⅱ方法是检测宫颈HPV感染的有效手段 ,HPV DNA含量随宫颈病变的严重程度而增加 ,可作为子宫颈癌的一种初筛方法。
Objective To explore the relationship between loads of human papillomavirus in cervical carcinoma and cervical intraepithelial neoplasia.Methods From December 2001 to May 2002, 9075 married women aged from 35 to 50 years who lived in the areas with a high incidence of cervical carcinoma of Shanxi Province were screened high risk types of human papillomavirus (HPV) infection using hybrid capture Ⅱ(HC-Ⅱ). Of them, 2087 women with positive human papillomavirus further underwent colposcopy and multi-focal directed punch biopsies plus endocervical currettage. Results Two thousand and eighty-seven women were found with positive human papillomavirus infection, comprising 1402 (67.2%) women who were diagnosed as cervicitis, 663 (31.8%) women diagnosed as cervical intraepithelial neoplasia [including cervical intraepithelial neoplasia (CIN) Ⅰ to Ⅲ], and 22 (1.1%) women diagnosed as cervical squamous cell carcinoma(SCC). HPV-DNA loads of women with chronic and acute cervicitis were 150±11 and 108±13, respectively, with no significant difference between two groups statistically (P=0.225). HPV-DNA loads for women with CIN Ⅰ, CIN Ⅱ and CIN Ⅲ were 332±29,358±35 , and 370±31, respectively ,all significantly higher than that of women with cervicitis (P=0.000), but there were no significant differences among three groups (P>0.05). HPV-DNA loads of women with cervical squamous cell carcinoma was 593±86 , much higher than those of women with varied grades of cervical intraepithelial neoplasia and cervicitis (P<0.05 and P<0.01).Conclusions The new technology using HC-Ⅱ is an effective method to detect HPV infection in cervix. Viral loads of HPV-DNA increase with severity of cervical neoplasia. So it could be used for screening primary cervical carcinoma.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第6期400-402,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
人乳头状瘤病毒
负荷量
子宫颈癌
癌前病变
Papillomavirus, human
Viral load
Cervix neoplasms
Precancerous conditions
