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褪黑素对大鼠骨髓基质干细胞诱导分化及抗凋亡作用的研究 被引量：2

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摘要 目的进一步探讨体外培养、扩增、纯化骨髓基质干细胞的方法，利用抗氧化剂诱导骨髓基质干细胞向神经元样细胞分化。方法利用全骨髓法培养成年SD大鼠骨髓基质干细胞。传至第4代，用褪黑素(10^-9mol/L)及全反式维甲酸(10^-6mol/L)诱导分化，于诱导后第5h行神经细胞特异性抗原MAP-2和胶质纤维酸性蛋白(GFAP)免疫组化鉴定，并于诱导后1、5、24h计数比较此两组神经元样细胞的分化率。结果神经元样细胞皆呈MAP-2阳性，GFAP阴性。褪黑素(10^-9mol/L)、RA(10^-6mol/L)诱导后第1h分化率分别为0．26、0．20；5h为0．56、0．53；24h为0．40、0．08。结论褪黑素可诱导骨髓基质干细胞分化为神经元样细胞；褪黑素可使神经元样细胞存活时问明显延长。
作者 张蒙夏 唐圣松 谢富康
机构地区 南华大学组织胚胎学教研室 南华大学药理学教研室 中山大学中山医学院组织胚胎学教研室
出处 《美国中华临床医学杂志》 2004年第1期51-53,58,共4页 American Journal of Chinese Clinical Medicine
关键词 褪黑素 大鼠 骨髓基质干细胞 细胞诱导分化 抗凋亡作用 MSC 神经组织
分类号 R331.22 [医药卫生—人体生理学]
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参考文献13

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共引文献5

同被引文献27

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引证文献2

二级引证文献12

美国中华临床医学杂志
2004年 第1期

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