摘要
目的 探讨在中性粒细胞弹力酶 (NE)诱导肺上皮细胞表达Elafin过程中核转录因子 κВ(NF κВ)的信号传导作用。方法 肺上皮细胞株A5 4 9传代培养后分 3组行条件孵育 ,分别为正常组、加精制猪胰NE孵育的对照组以及同时加酶和NF κВ抑制剂二硫代氨基甲酸吡咯烷(PDTC)孵育的实验组。孵育 2h后的细胞涂片行免疫荧光细胞染色法检测NF κВ的表达 ,酶联免疫吸附法 (ELISA)检测孵育 2 4h上清液的Elafin水平。结果 加入NE孵育的细胞上清液中的Elafin和NF κВ的表达强度与空白对照组比较均有显著性差异 (P <0 0 1) ;同时加入NE和PDTC孵育细胞的上述指标与NE组比较也存在显著性差异 (P <0 0 5 )。培养细胞NF κВ的表达强度与上清液中的Elafin呈显著正相关 (r=0 6 6 ,P <0 0 1)。结论 NE诱导肺上皮细胞Elafin表达的作用可能是通过激活NF
Objective To explore the role of nuclear factor-κВ (NF-κВ) in the signal transduction pathway of neutrophil elastase(NE) induced elafin expression in pulmonary epithelium.Methods Cultivated alveolar type Ⅱ cell line A549 were divided into 3 groups,ie.the normal group,the control group stimulated with NE and the experimental group stimulated with NE and pyrrolidine dithilcaramate (PDTC).The expression of NF-κВ was assayed by immunocytofluorescence staining and elafin in culture supernatants was measured by ELISA.Results The intensity of NF-κВ expression and the concentration of Elafin in supernatants of control group were significantly higher than those in normal group and experimental group(P<0.01)in dicating NF-κВ expression and Elafin production were significantly inhibited by PDTC.There was good correlation between the intensity of the NF-κВ expression and elafin concentration (r=0.66,P<0.005).Conclusion The active NF-κВ may play an important role in the NE-inducible production of elafin in pulmonary epithelium.
出处
《中国呼吸与危重监护杂志》
CAS
2004年第4期245-247,共3页
Chinese Journal of Respiratory and Critical Care Medicine
