摘要
目的 :探讨榄香烯抑制人喉肿瘤细胞 (HEp 2 )过程中与凋亡相关的Caspase 3活性的改变 ,以及真核细胞翻译起始因子 (eIF)家族成员 (eIF4E、eIF4G等 )和与肿瘤新生血管有关的碱性纤维母细胞生长因子 (bFGF)、血管内皮生长因子 (VEGF)的表达。方法 :使用比色法 ,研究榄香烯诱导人喉鳞状细胞癌细胞株HEp 2细胞凋亡时Caspase 3活性的变化 ;并使用Westernblot和RT PCR法检测榄香烯作用的HEp 2细胞中eIF4E、eIF4G、bFGF、VEGF在mRNA和蛋白水平的表达。结果 :Caspase 3活性在药物作用 12h达到最高 ,并随药物作用时间的延长而下降 ;随着榄香烯作用时间的延长 ,eIF4E、eIF4G、bFGF、VEGF蛋白表达下降 ,bFGF、VEGF的mRNA含量亦下降。结论 :榄香烯抑制HEp 2细胞生长可引起Caspase 3活性的改变 ;eIF4E、eIF4G的表达下降与bFGF、VEGF的表达呈正相关 ;bFGF、VEGF表达的下降与eIF家族成员表达的下降所引起的翻译水平的抑制有关。
Objective:To investigate the activity of Caspase 3 and the expression of eukaryotic initiation factor families, bFGF and VEGF after elemene on laryngeal carcinoma HEp 2 cells.Method:The HEp 2 cells after elemene treatment were analyzed utilizing Westernblot and reverse transcriptase polymerase chain reaction (RT PCR). The activity of Caspase 3 was assessed by colorimetric assay.Result:The activity of Caspase 3 was enhanced after elemene treatment.The protein expression of eIF4E, eIF4G, bFGF and VEGF were significantly inhibited by elemene; and the mRNA expression of bFGF and VEGF were inhibited either.Conclusion:Elemene can effectively inhibit the growth of HEp 2 cells and result in the alteration of activity of Caspase 3. There were significant correlations between the decreased expression of protein eIF4E, eIF4G, bFGF and VEGF. The mechanism of eIF4E and eIF4G decrease the expression of bFGF and VEGF is post transcriptional.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2004年第6期367-369,375,共4页
Journal of Clinical Otorhinolaryngology
基金
上海市科技发展基金资助项目 (No:0 14 1190 4 7)
