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壬基酚对鲫鱼(Carassius auratus)的雌激素效应研究 被引量:25

The Estrogenic Effect of 4-nonylphenol on the Synthesis of Vitellogenin in Carassius auratus
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摘要 实验研究了环境雌激素4-壬基酚(NP)对鲫鱼(Carassiusauratus)的剂量-效应关系。雄性鲫鱼腹腔注射不同剂量的17β-雌二醇(E2)(质量分数分别为0 01,0 10,1 00mg kg(注射剂量 鱼体重,下同))和4-壬基酚(NP)(质量分数分别为1,10,50,100,200mg kg),用ELISA方法测定鲫鱼血浆中卵黄蛋白原含量。结果显示,与对照相比,在注射后14d内,0 01mg kgE2处理组和1 00mg kgNP处理组鲫鱼血浆卵黄蛋白原含量没有显著差异;其他各处理组在注射后8d,血浆卵黄蛋白原含量显著升高(P<0 05),且与暴露浓度呈依赖关系;注射后14d,10,50,100和200mg kgNP处理组的含量依次是对照组的10,59,340和435倍。研究表明,NP对鲫鱼具有明显的雌激素效应。 Dose response effects of the putative environmental estrogen 4-nonylphenol(NP) were investigated in the Carassius auratus.Male Carassius auratus received intraperitoneal injections with a dose series of 17β-estradiol(E_2)(0.01,0.10 and 1.00 mg/kg; single injection) and 4-NP(1,10,50,100 and 200 mg/kg;single injection).The estrogenic effects of the treatment were investigated on vitellogenin measured by enzyme linked immunosorbent assay(ELISA) at different time points.There was no significant increase in the plasma vitellogenin concentration in 0.01 mg/kg of E_2 treatment group as well as in 1.00 mg/kg of NP during 14 d.8 d after injection,a significant increase(P<0.05) in the plasma vitellogenin concentration was observed in all the other treatment groups.14 d after injection,the vitellogenin concentration in 10,50,100 and 200 mg/kg of NP treatment groups were respectively raised to 10,59,340 and 435-fold,compared to controls.These results demonstrated that NP possesses estrogenic activity.
出处 《环境科学研究》 EI CAS CSCD 北大核心 2004年第3期60-61,70,共3页 Research of Environmental Sciences
基金 上海市科委重大项目(002312015) 上海市重点学科生态学建设项目
关键词 4-壬基酚 鲫鱼 卵黄蛋白原 nonylphenol Carassius auratus vitellogenin
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参考文献11

  • 1Harries J E,Sheahan D A.Oestrogenic activity in five United Kingdom rivers detected by measurement of vitellogeninesis in caged male trout[J].Environ Toxicol Chem,1997,16:534-542.
  • 2Livingstone D R,Mitchelmoe C L,Pters L D, et al.Development of hepatic CYP1A and blood vitellogenin in eel(Anguilla anguilla) for use as biomarkers in the Thames Esturary,UK[J].Marine Environ Res,2000,50:367-371.
  • 3Kime D E,Nash J P,Scott A P.Vitelloenesis as a biomarker of reproductive disruption by xenobiotics[J].Aquaculture,1999,177:345-352.
  • 4Jobling S,Sumpter J P.Detergent components in sewage effluent are weakly oestrogenic to fish:An in vitro study using rainbow trout(Oncorhynchus mykiss) hepatocytes[J]. Aquatic Toxicol,1993,27:361-372.
  • 5Jobling S,Sheahan D,Osborne J A,et al.Inhibition of testicular growth inrainbow trout Oncorhynchus mykiss exposed to estrogenic alkylphenolic chemicals[J]. Environ Toxicol Chem,1996,15:194-202.
  • 6Christiansen T,Korsgaard B,Jespersen A.Effects of nonylphenol and 17b oestradiol on vitellogenin synthesis,testicular structure and cytology in male eelpout Zoarces viviparus[J]. Journal of Experimental Biology,1998,201:179-192.
  • 7Yadetie F,Arukwe A,Goksoyr A,et al.Induction of hepatic estrogen receptor in juvenile Atlantic salmon in vivo by the environmental estrogen,4 nonylphenol[J].Sci Total Environ,1999,233:201-210.
  • 8Ishibashi H,Tachibana K,Tsuchimoto M,et al.In vivo testing system for determining the estrogenic activity of endocrine disrupting chemicals(EDCs)in goldfish(Carassius auratus)[J].J Health Sci,2001,47:213-218.
  • 9Ahel M,Giger W,Koch M.Behaviour of alkylphenol polyethoxylate surfactants in the aquatic environmentⅠ.Occurrence and transformation in sewage treatment[J].Water Res,1994,28:1131-1142.
  • 10Christensen L J,Korsgaard B,Bjerregaard P.The effect of 4 nonylphenol on the synthesis of vitellogenin in the flounder Platichthys flesus[J].Aquatic Toxicol,1999,46:211-219

二级参考文献10

  • 1李康 王明山 周忠良.鲫鱼(Carassius auratus)卵黄蛋白原的诱导[J].上海师范大学学报,2001,12:42-47.
  • 2Sherry J, Gamble A, Fielden M, et al. An ELISA for brown trout(Salmo trutta) vitcllogenin and its use in bioassays for environmental estrogens [J]. The Science of the Total Environment,1999,225:13-31.
  • 3Lowry O H, Rosebrough N J, Farr A L, et al. Protein measurement with a Folin reagent [J]. Journal of Biological Chemistry, 1951,193:265-275.
  • 4Mommsen T P, Walsh P J. Vitellogenesis and oocytc assembly[A]. Hoar W S, Randall D J, Eds. Fish physiology volume XI,the physiology of developing fish part a, eggs and larvae [M].London: Academic Press, 1988.347-406.
  • 5Kime D E, Nash J P, Scott A P. Vitellogenesis as a biomarker of reproductive disruption by xenobiotics [J]. Aquaculture, 1999,177:345-352.
  • 6Nicolas J M. Vitcllogcncsis in fish and the effects of polycyclic aromatic hydrocarbon contaminants [J]. Aquatic Toxicology,1999,45:77-99.
  • 7Hashimoto S, Bessho H, Hara A, et al. Elevated serum vitellogenin levels and gonadal abnormalities in wild male flounder (Pleuronectes yokohamae) from Tokyo Bay, Japan [J].Marine Environmental Research, 2000,49:37-53.
  • 8Livingstone D R, Mitchelmore C L, Peters L D, et al.Development of hepatic CYP1A and blood vitellogenin in eel(Anguilla anguilla) for use as biomarkers in the Thames Estuary,UK [J]. Marine Environmental Research, 2000,50:367-371.
  • 9Bon E, Barbe U, Nunez Rodriguez J, et al. Plasma vitellogenin levels during the annual reproductive cyle of the female rainbow trout (Oncorhynchus mykiss): establishment and validation of an ELISA [J]. Comparative Biochemistry and Physiology, 1997,117B:75-84.
  • 10Lomax D P, Roubal W T, Moore J D, et al. An enzyme-linked immunosorbent assay (ELISA) for measuring vitellogenin in English sole (Pleuronectes vetulus): development, validation and cross-reactivity with other pleuronectids [J]. Comparative Biochemistry and Physiology, 1998,121 B:425-436.

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