摘要
目的 研究探讨RhoC基因mRNA和蛋白的过量表达与肝细胞癌 (HCC)的发生发展和侵袭转移的关系。方法 采用逆转录聚合酶链反应 (RT PCR)和Western印迹法 ,检测 2 5例HCC及其癌旁肝组织、4例门静脉主干癌栓或肝外转移灶中RhoCmRNA和蛋白的表达 ;同时采用PCR产物单链构象多态性 (PCR SSCP)银染法检测RhoC基因的突变情况。结果 HCC组织和癌旁肝组织中均可检测到RhoC基因mRNA和蛋白的表达 ,RhoCmRNA在HCC组织中的表达较癌旁肝组织高 ,其吸光度(A)比值分别为 1.8± 1.1和 1.0± 0 .7,差异有显著性 (P <0 .0 1) ;RhoC蛋白在HCC组织中的表达同样高于癌旁肝组织 ,其A比值分别为 33992± 10 384和 17342± 9998,差异有显著性 (P <0 .0 1)。 4例门静脉主干癌栓或肝外转移灶中的RhoC基因mRNA和蛋白的表达量分别为 3.3± 0 .5和 6 386 5±9116 ,较HCC肝内病灶高 ,差异有显著性 (P <0 .0 1) ;RhoCmRNA和蛋白在分化较差、结节数较多、有静脉浸润或伴有肝外转移灶的HCC中过量表达 (P <0 .0 5 )。全部HCC经PCR SSCP银染法分析均未发现异常泳动条带。结论 RhoC基因的过量表达与HCC的发生发展和侵袭转移密切相关 ,且可能是通过过量表达而非突变发挥作用。
Objective Rho,a ras homologous gene, encodes a group of GTP-binding proteins. Our previous study suggested that one member of the Rho gene family,RhoC,was related to the progression of human hepatocellular carcinoma (HCC). This study is to elucidate correlation of Rho overexpression with invasion and metastasis of HCC. Methods The expression level of RhoC mRNA and protein in 25 cases of HCC and adjacent non-cancerous liver tissue was examined by RT-PCR and Western blot. Mutation of RhoC gene was examined by PCR-SSCP. Results The expression of RhoC mRNA and protein was found in all HCC and adjacent non-cancerous liver tissue. The expression level of RhoC mRNA and protein was significantly higher in tumor tissue than in adjacent non-cancerous liver tissue (1.8±1.1 vs 1.0±0.7; 33 992±10 384 vs 17 342±9998, P< 0.01). The degree of RhoC overexpression was even more marked in metastatic lesions than in primary tumors (P<0.01). Overexpression of the rhoC gene was significantly correlated with such clinic-pathological findings as cell differentiation, portal vein invasion, number of primary tumor nodules and metastatic lesions (P<0.05). Mutation of RhoC gene was found in none of the HCC specimens examined. Conclusion Overexpression of RhoC gene may play an important role in carcinogenesis and progression of HCC.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2004年第5期279-282,共4页
Chinese Journal of Oncology
基金
国家"十五"科技攻关项目 (2 0 0 1BA70 3B0 4)
湖南省计委资助项目 (2 0 0 1 90 7)
