摘要
目的 探讨神经生长因子 (NGF)对颅脑损伤后神经细胞的保护作用。方法 建立大鼠液压脑损伤模型 ,向其脑室灌注 7SNGF 5 μg ,观测其对脑组织超氧岐化酶 (SOD)、谷胱苷肽过氧化物酶 (GSH Px)和过氧化物酶 (CAT)活性、[Ca2 + ]i及细胞凋亡的影响。结果 (1)伤后 3d ,NGF处理组SOD、GSH Px和CAT含量分别为 (198.96± 7.76)UN/ml、(1.2 1± 0 .0 4)UN/mg蛋白、(3 2 .5 8± 2 .0 1)UN/mg蛋白 ,显著高于单纯致伤组 (P <0 .0 1)。 (2 )伤后 2 4h ,NGF处理组 [Ca2 + ]i为 (878.5 6± 13 7.3 0 )nmol/L ,明显低于单纯致伤组 (972 .48± 14 6.66)nmol/L(P <0 .0 1)。 (3 )NGF处理组凋亡细胞显著少于单纯致伤组 (P <0 .0 1)。结论 NGF能增加颅脑损伤后脑组织中主要抗氧化酶的活性 ,降低 [Ca2 + ]i超载 ,对神经细胞有明显保护作用。
Objective To investigate the protective effect of nerve growth factor (NGF) on neurons after traumatic brain injury (TBI).Methods A brain trauma model of fluid-percussion in rats was established and 5 μg 7sNGF was infused in its cerebral ventricle.Activity of SOD,GSH-Px and CAT,i,and neuron apoptosis in brain tissues were observed.Results Activities of SOD,GSH-Px and CAT were markedly higher in NGF treatment group than in simple trauma group on the 3rd posttrauma day,which were (198.96±7.76) UN/ml,(1.21±0.04) UN/mg.protein and (32.58±2.01) UN/mg. protein respectively ( P <0.01).The level of i in NGF treatment group (878.56± 137.3) ?nmol/L was significantly lower than in simple trauma group (972.48±146.66)?nmol/L at 24th h after injury ( P <0.01).The number of apoptosis neurons was obviously lower in NGF treatment group than in simple trauma group ( P <0.01).Conclusion After traumatic brain injury,exogenous NGF can increase the activity of the major antioxidant enzymes in brain tissues and reduce the severe overload of i and provide obvious protective effects against apoptosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第5期588-589,共2页
Chinese Journal of Experimental Surgery
