摘要
目的 探讨中药复方益肝康抗肝纤维化的作用机制。方法 给正常大鼠灌服益肝康 ,提取药物血清 ,温育体外培养的肝星状细胞。3H TdR掺入法测定细胞增殖 ,流式细胞技术分析细胞周期 ,透射电镜观察细胞超微结构 ,免疫细胞化学法检测PDGF BB蛋白表达。结果 益肝康药物血清显著抑制肝星状细胞的增殖 ,可使细胞生长周期延长 ,细胞增殖、活力受抑 ,并明显抑制PDGF BB蛋白表达。结论 益肝康可抑制肝星状细胞增殖 ,对PDGF
Objective To investigate the main seropharmacological mechanisms in the antifibrotic action of Yigankang decoction. Methods After administration of normal rats with Yigankang decoction through the stomach, rat serum was collected for incubation with the hepatic stellate cells (HSCs) cultured in vitro . The proliferation of HSCs was measured by 3H TdR incorporation. Cell cycles of HSCs were detected by flow cytometry. The ultrastructures of HSCs were observed transmission electron microscopically. The protein expression of platelet derived growth factor BB (PDGF BB) was determined by immunocytochemistry. Results Serum of rats after administration of Yigankang decoction could obviously inhibit the proliferation of HSCs, increase the proportion of G 0/G 1 stage, and down regulate PDGF BB protein expression. Conclusion The inhibitory effect of Yigankang decoction on the proliferation of HSCs and PDGF BB expression may be one of the main mechanisms in the antifibrotic action.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第7期611-613,共3页
Journal of Third Military Medical University
关键词
益肝康
肝纤维化
肝星状细胞
血小板衍生生长因子
Yigankang decoction
hepatic fibrosis
hepatic stellate cell
platelet derived growth factor BB
