摘要
采用95%乙醇对杠柳(PeriplocasepiumBunge)根皮进行热提取,以叶片浸渍法和点滴法测定了提取液对小菜蛾(PlutellaxylostellaL.)的杀虫活性及其作用方式。结果显示,杠柳乙醇提取液稀释100倍处理对小菜蛾3龄和4龄幼虫24h后的非选择性拒食率分别为87.3%和96.3%;100倍液浸叶饲喂处理对小菜蛾2龄幼虫72h后的校正死亡率为80%,对小菜蛾3龄幼虫24h和48h后的生长抑制率为100%。杠柳乙醇提取液对小菜蛾幼虫具有较高的生物活性,其作用方式包括拒食作用、胃毒作用和生长抑制作用。此外,乙醇提取液对小菜蛾幼虫还有一定的触杀和内吸效应,并对小菜蛾成虫产卵有明显的忌避活性,但对小菜蛾卵没有杀伤作用。
The root barks of Periploca sepium Bunge were hot-extracted with 95% ethanol, and leaf immersion method and topical application were used to test the bioactivity of the extracts against Plutella xylostella L. The results indicated that antifeeding rate was 87.3% and 96.3% against 3rd and 4th instar larvae of P. xylostella after 24 h of treatment with 100 times dilution of the ethanol extracts in no-choice test respectively. The corrected mortality of the larvae within 72 h reached 80% when the 2nd instar larvae of P. xylostella were fed by the leaf discs treated with 100 times dilution of the extracts. The growth inhibition rate of 100 times dilution of the extract was 100% to 3rd instar larvae. It is clear that the ethanol extracts has strong antifeeding property, stomach poison and growth inhibition to larvae of P. xylostella. In addition, the ethanol extracts of P. sepium possesses certain contact poison and systemic toxicity on the insect. The experimental results also showed that the crude extracts had obvious deterrent effect against the oviposition of P. xylostella. However, the extracts showed no killing effect on the eggs of P. xylostella.
出处
《植物资源与环境学报》
CAS
CSCD
2004年第3期31-34,共4页
Journal of Plant Resources and Environment
基金
山西省农业科技攻关资助项目(971011)
山西省青年基金资助项目(971033)
