摘要
为研究SARS冠状病毒的RNA干涉,以S蛋白为目标选取16个RNA干涉的靶序列,并设计用于体内转录形成以U6为启动子的siRNA发夹结构的DNA,拟将设计的DNA瞬时转染靶细胞,用定量RT-PCR法确定目标RNA被干涉的程度,用Westernblot在蛋白质水平上进行监测。针对SARS冠状病毒的RNAi设计为进一步研究奠定了理论基础,其工作的开展将在RNAi治疗、SARS冠状病毒基因功能研究、新药开发等方面发挥重要作用。
For studying the RNA interference to the S protein of SRAS coronavirus,16siRNA candidate targets were se-lected focusing on S protein.The hairpin DNA was designed for forming siRNA in vivo transcription.The designed DNA would be transfected into target cells.The real time RT-PCR and Western blot would be adopted for the identification of RNAi.The RNAi design to the S protein of SARS coronavirus laied the theory foundation for the further experimental re-searches.The researches on RNAi would make the important effect on the RNAi treatment,the function study of gene and drug design for SARS coronavirus.
出处
《生物技术通讯》
CAS
2004年第4期335-337,共3页
Letters in Biotechnology
