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猪瘟病毒C株兔脾毒在SK6细胞中的增殖培养及其鉴定

The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection
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摘要 Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6. Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6.
作者 张淼涛 冯霞 刘湘涛 张彦明 谢庆阁
机构地区 中国农业科学院兰州兽医研究所畜禽疫病重点实验室 西北农林科技大学
出处 《中国病毒学》 CSCD 2004年第4期404-406,共3页 Virologica Sinica
基金 国家重点基础研究发展规划(G19990119)
关键词 猪瘟病毒 CSFV C株 增殖培养 鉴定 变异 Classical swine fever virus C-strain Multiplication culture Detection.
分类号 S852.65 [农业科学—基础兽医学]
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参考文献4

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二级参考文献11

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共引文献20

中国病毒学
2004年 第4期

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