摘要
克隆得到 2种缘毛类纤毛虫———钟形钟虫 (Vorticellacampanula)和螅状独缩虫 (Carchesiumpolyp inum)的胞质Hsp70基因部分序列 ,长度均为 4 38bp ,编码 14 6个氨基酸。以细菌为外类群 ,利用最大似然法和邻接法构建包括其他 5种纤毛虫在内的共 2 6个物种的Hsp70基因氨基酸序列系统发育树 ,其拓扑结构显示 :V campanula和C polypinum聚在一起 ,并与另 2种寡膜纲的嗜热四膜虫 (Tetrahymenathermophila)及草履虫(Parameciumtetraurelia)聚为姊妹枝 ,提示了缘毛类纤毛虫为单系 ,且隶属于寡膜纲的系统发育地位。
Cytosolic Hsp70 genes of two peritrichous ciliates (Vorticella campanula & Carchesium polypinum) were partially sequenced and the lengths are 438 bp,both encoding 146 amino acids.Using bacteria as the outgroup,the phylogenetic trees were constructed based on cytosolic Hsp70 amino acid sequences by maximum-likelihood (ML) and neighbor-joining (NJ) methods.The topology that V.campanula clustered with C.polypinum,and then formed a sister clade with another two species in Oligohymenophorea (Tetrahymena thermophila & Paramecium tetraurelia),suggested the monophyly of the peritrichs and their phylogenetic placement in the class Oligohymenophorea.
基金
中国科学院创新工程基金资助项目 ( 2 2 0 2 0 7)
东湖湖泊生态系统试验站开放基金资助项目 ( 2 0 0 40 5 )
