摘要
根据GenBank上登录的堆形艾美球虫Ea1A基因序列 ,设计了 1对引物 ,以抽提的广东株的总RNA为模板 ,利用反转录 聚合酶链反应 (RT PCR)扩增获得了Ea1A基因部分片段 ,并将此片段克隆至pGEM T载体中 ,经PCR、限制性内切酶分析和克隆片段序列测定、比较 。
Based on Ea1A nucleotide sequence of E.acervulina published, a pair of primers were (designed). Using the total RNA of the strain Guangdong of E.acervulina, which was isolated from an outbreak in Guangdong Province, as a template, a partial segment of Ea1A was amplified by RT-PCR. The Ea1A gene fragment was cloned into pGEM-T Easy vector, and the recombinant plasmid was named pGEM-Ea1A. Finally, the plasmid was identified by PCR, restriction enzyme analysis and sequencing.
出处
《中国兽医科技》
CAS
CSCD
北大核心
2004年第8期44-47,共4页
Chinese Journal of Veterinary Science and Technology
