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杀鲑气单胞菌的快速检测 被引量:5

Rapid Detection of Aeromonas salmonicida Using ELISA
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摘要 杀蛙气单胞菌引起的鱼类疖疮病是进口动物检疫对象。该菌只能在疾病流行期从病鱼体内分离。在水和鱼卵中虽有此菌,也无法用常规细菌分离和培养法进行分离和鉴定。用ELISA技术快速检测水样中的该菌获得成功,为进口虹鳟鱼卵时检测该菌提供了方法。 Aeromonas salmonicida causing furunculosis of fish and other fish diseases could only be isolated from fish during diseas period. It is also difficult to isolate and identify this organism with routine bacteriological isolation and culture though it is present in water and roes. We have tried EL1SA in detection of Aeromonas salmonicida in water with success, the detectable level could reach 5 organisms/ml providing that 100ml water sample containing 500 organisms could be concentrated into 0. 05ml. The experiment results showed that EL1SA is a simple and quick technique in the detection of Aeromonas salmonicida in water with high sensitivity and specificty.
出处 《动物检疫》 1993年第5期7-8,共2页
关键词 杀鲑气单胞菌 检测 检疫 Aeromonas salmonicida, ELISA
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