摘要
目的 探讨TGF β1 (transforminggrowthfactorβ1 、TGF β1 )、TGF βRⅠ、Ⅱ (TGF βRTypeⅠ ,Ⅱreceptor,TGF βRⅠ、Ⅱ )在人类各种肾小球疾病中的作用及与凝血纤溶因子 (FRA、FN、α2 PI、PLG)之间的相互关系及其促使肾小球硬化的作用机理 ,从而寻找影响肾小球疾病发生发展的主要因素。方法 采用免疫组化SABC法检测系膜增生性肾炎 (MsPGN)、系膜毛细血管性肾炎(MPGN)、局灶节段性肾小球硬化 (FGS)、膜性肾病 (MN)共 4 1例中TGF β1 、TGF βRⅠ、Ⅱ的表达 ;直接免疫荧光法检测FN和FRA ;间接免疫荧光法检测α2 PI和PLG ;共 6 5例。全部数据采用Ridit分析、Spearman’s等级相关。结果 (1 )TGF β1 、TGF βRⅠ、Ⅱ与FRA、FN、α2 PI、PLG之间存在显著正相关 ,随肾组织炎症程度及纤维化程度加重 ,其表达增强。 (2 )肾小球纤维化是多因素共同作用的结果。其中TGF β1 、TGF βRⅠ、Ⅱ与FRA、FN起着非常重要的主因素作用。 结论 TGF β1 、TGF βRⅠ、Ⅱ与FRA、FN、α2 PⅠ、PLG在肾组织内的共同表达及协同作用 。
Objective To explore the expressions of transforming growth factor β 1 (TGF β 1), and its types Ⅰ and Ⅱ receptors (TGF β Type Ⅰ, Ⅱ Receptors, TGF βRⅠ, Ⅱ), and their roles and relationships in the mechanism of human glomerulonephritis, their correlation with blood coagulation fibrinolysis factors (FRA, FN, α 2 PI, PLG), and the accelerative effects on glomeruli cirrhosis,so as to identify the key factor that triggers and develops human glomerulonephritis.Methods Through immunohistochemistry, SABC, to assay the reflected changes in glomerular expressions of TGF β 1 and TGF βRⅠ and Ⅱ, and in selected biopsy samples of the following 41 disease cases By applying direct immunofluorescence to assay FN and FRA,indirect immunofluorescence assays α 2 PI, and PLG, in 65 disease cases.Statistical analysis through Ridit and Spearman's analysis.Results (1)Significant close correlation was noted between(TGF β 1,TGF βRⅠ andⅡ),and(FRA、FN、α 2 PI、PLG)in the sampling disease cases,and the expressions significantly increase in direct correlation to the increased severity of renal tissue inflammation and fibrosis.(2)The glomerular fibrosis is the combined effect of several factors.The key factors include(TGF β 1,TGF βRⅠ,Ⅱ)and(FRA,FN).Conclusion The expressions and correlations within renal tissues of(TGF β 1,TGF βRⅠ and Ⅱ),and(ERA、FN、α 2 PI、PLG),indicate these are significant factors in the developments of renal tissue fibrosis or cirrhosis.
出处
《中国血液流变学杂志》
CAS
2004年第2期153-156,共4页
Chinese Journal of Hemorheology
