摘要
目的 :了解河豚肝脏提取物多肽A、B、C的抗瘤活性。方法 :体外细胞毒试验是用噻唑蓝 (MTT)法。体内抗瘤试验是用小鼠肿瘤模型S 180肉瘤。结果 :多肽A在 12 5～ 10 0 0mg·L-1浓度下 ,对人鼻咽癌细胞 (CNE2 )、人肝癌细胞 (Bel 74 0 2 )、人肺腺癌细胞 (GLC 82 )和人白血病细胞 (K 5 6 2 )的半数抑制浓度分别为 341.5 5、6 0 7.96、5 2 0 .82mg·L-1和 833.13mg·L-1;而对人胚肾上皮细胞 (HK 2 93)、人脐血管内皮细胞 (EVC 4 0 3)和人体肝细胞 (ChangLiver)的IC50 分别为 6 2 6 .2 2、5 74 13、4 93.4mg·L-1。而多肽B、C对上述细胞的生长均无作用。体内抗瘤试验表明 ,多肽A用 6 0～ 180mg·(kg·d) -1,ipqd× 10d ,对小鼠肿瘤S 180肉瘤的抑制率为 4 2 .0 %～ 4 5 .2 % ,与对照组比较差异有显著性 (P <0 .0 5 )。结论 :河豚肝脏提取物多肽A在体内、体外试验有一定的抗瘤活性 ,但其抗瘤作用与剂量无相关性 。
Objective: to investigate the antitumor activity of polypeptides extracted from hepar of puffer fish. Methods: cytotoxicity test in vitro were measured by MTT assay. In vivo antitumor test used were models of mice S-180 sarcoma. Results: under the 125～1000 mg·L -1 of polypeptides,the fifty percent inhibition concentration(IC 50) on human nosopharyngeal carcinoma cell line(CNE2), human liver carcinoma cell line(Bel-7402), human lung adenocarcinoma cell line(GlC-82),and human leukemia cell line(K-562) were 341.55 mg·L -1, 607.096 mg·L -1, 520.82 mg·L -1,and 833.13 mg·L -1,respectively . The IC50 value on human embryonic kidney cellline (HEK-293), and Chang Liver cells(CLC), were 626.32 mg·L -1, 574.13 mg·L -1 and 493.4 mg·L -1, respectively. In vivo experiment showed that the tumor inhibitory rates of polypeptides A were 42.0～45.2%(P<0.01), against S-180 sarcoma, under the doses of 60-180 mg·(kg·d) -1, intraperitoneal (ip) qd×10d. but polypeptides B and C showed noantitumor effects. Conclusions: the results suggested that the polypeptides A possess antitumor effects in vitro and in vivo, but is not related to doses of polypeptides A, and exhibit noselective inhibition on the human cancer cell and human normal cell.
出处
《广东药学院学报》
CAS
2004年第3期278-280,共3页
Academic Journal of Guangdong College of Pharmacy
关键词
河豚提取物
多肽A
抗瘤活性
polypeptides A
extracts of puffer fish
antitumor activity
