摘要
目的 :探讨涎腺粘液表皮样癌中血管内皮生长因子 (vascularendothelialgrowthfactor ,VEGF)的表达及与血管生成、肿瘤细胞增殖的关系。方法 :应用免疫组织化学SP法检测 2 8例涎腺粘液表皮样癌组织中VEGF、PCNA的表达 ,用CD34标记血管内皮细胞计数肿瘤内微血管密度 (MVD) ,并进行统计分析。结果 :本组病例VEGF阳性表达率为 82 .14 % (2 3/ 2 8) ;MVD为 30 .19± 12 .99(8～ 5 9) ,PCNA为 (2 0 .5 0± 12 .5 8) % (6 .2 3%～5 1.78% )。低分化组MVD、PCNA阳性率均高于高分化组 (P <0 .0 5 )。VEGF表达阳性的肿瘤组织MVD高于阴性者 (P <0 .0 5 ) ,VEGF表达与MVD呈正相关 (rs=0 .80 ,P <0 .0 5 )。VEGF表达、MVD与PCNA表达均无明显相关性 (相关系数分别为rs=0 .0 2 ,rs=0 .15 ,P >0 .0 5 )。结论 :涎腺粘液表皮样癌中 ,肿瘤细胞能产生VEGF促进血管生成 ;低分化粘液表皮样癌微血管密度及肿瘤细胞增殖活性均高于高分化粘液表皮样癌。
Objective: To evaluate the expression of vascular endothelial growth factor(VEGF) and its relationship with angiogenesis and tumor cell proliferation in mucoepidermoid carcinoma(MEC) of salivary glands. Methods: Expression of VEGF,intratumoral microvessel density (MVD,labeled by anti-human CD34 monoclonal antibody) and tumor cell proliferation( labeled by proliferating cell nuclear antigen,PCNA) in 28 cases of mucoepidermoid carcinoma of salivary glands was examined immunohistochemically. Results: Positive staining for VEGF was obtained in 23 of the 28 cases (82.14%). MVD varied from 8 to 59(30.19±12.99).The percentage of PCNA po sit ive cells varied from 6.23% to 51.78%\.Both MVD and the percentage of PCNA positive cells were significantly higher in high-grade cases than in low-grade cases( P < 0.05 ).MVD was significantly higher in VEGF positive than in VEGF negative cases( P < 0.05) . The correlation between the expression of VEGF and MVD was significant( r _s=0.80, P < 0.05 ).No correlation was found between the expression of VEGF or MVD and the percentage of PCNA po sit ive cells ( r _s=0.02,r _s=0.15,respectively,P >0.05).Conclusion: In MEC of salivary glands,tumor cells can produce VEGF to stimulate angiogenesis;both MVD and the percentage of PCNA positive cells is significantly higher in high-grade MEC than in low-grade MEC.
出处
《武汉大学学报（医学版）》
CAS
2004年第4期426-428,403,F003,共5页
Medical Journal of Wuhan University
关键词
涎腺粘液表皮样癌
血管内皮生长因子
血管生成
增殖
mucoepidermoid carcinoma
vascular endothelial growth factor
angiogenesis
proliferation
