摘要
目的探讨急性砷中毒小鼠血清中一氧化氮(NO)、还原型谷胱甘肽(GSH)、丙二醛(MDA)及总抗氧化能力的变化,及其在砷中毒发病机制中的作用。方法通过急性动物实验,小鼠灌胃染毒,分为低、中、高剂量组及对照组,采用生化试剂盒测定各组小鼠血清中NO、GSH、MDA及总抗氧化能力。结果低剂量组小鼠血清中NO、GSH显著高于对照组(P<0.05),高剂量组NO、GSH则低于对照组(P<0.05);各剂量组NO、GSH和总抗氧化能力随染砷剂量的增加而降低,MDA水平则显著增高(P<0.01)。结论三氧化二砷(As2O3)可引起小鼠血清中GSH、MDA和总抗氧化能力发生改变,进而影响NO合成与代谢,导致脂质过氧化的发生。提示脂质过氧化可能是砷中毒的机理之一。
Objective To clarify the variety and the significance of nitric oxide(NO), glutathione(GSH), maleic dialdehyde(MDA) and total anti-oxidize ability in serum of mice with acute arsenic poisoning. Methods Animal experiment and biochemistry agent box of NO, GSH, MDA and total anti-oxidize ability were used. Results The contents of NO and GSH in low dose groups were higher than those in controls in serum of mice(P < 0.05), and decreased in high dose group. NO, GSH and total anti-oxidize ability in all experimental groups decreased and showed a dose-effect manner. MDA in all groups increased significantly and showed a dose-effect manner(P < 0.01). Conclusions The changes of GSH, MDA and total anti-oxidize ability could be affected by As2O3. The synthesis or metabolize of NO may be correlated with the influence of arsenite on GSH, MDA and total anti-oxidize ability. The results indicates that arsenic could induce lipid peroxidation (LPO) in mice. LPO might be one of the mechanism of arsenic poisoning.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2004年第4期325-326,共2页
Chinese Jouranl of Endemiology
