摘要
目的 :研究阳离子脂质体 (cationicliposome)介导甲胎蛋白 (α fetoprotein ,AFP)启动子 /增强子载体在分泌AFP的人肝癌细胞株中的靶向表达。方法 :测定比较阳离子脂质体Lipofectin介导质粒pDR2 luc、pEBAFluc转染分泌AFP的人肝癌细胞株 (HepG2 )、不分泌AFP的人肝癌细胞株 (SMMC772 1 )、人肾癌细胞株 (GRC)及非洲绿猴肾细胞(COS7)的转染效率 ,通过液体闪烁计数仪单光子计数法测定荧光素酶活性 ,估计转染效率。结果 :①以Lipofectin介导pDR2 luc转染 4株细胞 ,荧光素酶活性在 4株细胞中均能表达 ,且表达活性差异有统计学意义 (P <0 .0 5 ) ;②以Lipofectin介导pEBAFluc转染 4株细胞 ,荧光素酶活性仅在能分泌AFP的HepG2 中表达。结论 :含人AFP启动子
Aim: To study the targeting effect to the α fetoprotein(AFP) positive human hepatocarcinoma cell line by the cationic liposome mediated by transfection of tissue specific vector. Methods: Plasmid pDR 2luc and pEBAFluc contained the firefly luciferase reporter gene under the control of the RSV LTR promoter and the AFP promoter, respectively. Four cell lines including human hepatocarcinoma cell line HepG 2and SMMC7721, human renal carcinoma cell line GRCand simian kidney cell line COS 7were used to be transfected by cationic liposome Lipofectin. The transfection efficiency was evaluated by the activity of expressed luciferase. Results: When transfected with lipofectin pDR 2luc, the Luciferase activity expressed in four cell lines and were statistically significant ( P < 0.05). When transfected with Lipofectin pEBAFluc, the luciferase activity only expressed in HepG 2, but did not expressed in the other three cell lines. Conclusion: pEBAFluc vector harboring AFP promoter/enhancer has targeting effect to the AFP positive hepatocarcinoma cells.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2004年第4期613-616,共4页
Journal of Zhengzhou University(Medical Sciences)
关键词
脂质体
甲胎蛋白
转录调控序列
癌
肝细胞
liposome
α fetoprotein
transcriptional regulatory sequences
carcinoma, hepatocellular
