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48家医院自身抗体检测的质控总结 被引量:17

Quality control of autoantibodies detection with analysis
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摘要 目的了解我国自身抗体的检测现状,提高现有的检测水平。方法通过发函的方式确定48家医院为参与单位,质控项目包括抗核抗体、抗dsDNA抗体、抗可提取性核抗原(ENA)抗体、抗线粒体抗体和抗平滑肌抗体。标本发放和结果分析采用双盲操作,质控标本共计11份。结果抗核抗体、抗dsDNA抗体、抗ENA抗体、抗线粒体抗体和抗平滑肌抗体正确率分别是47%、70%、46%、95%和40%。结论总体情况不容乐观,试剂盒质量参差不齐,检测人员水平不一。 Objective To make an investigation for autoantibodies detection in China. Method Forty-eight hospitals were included by mail or Email. The items of QC include ANA, anti-ENA antibody, anti-dsDNA antibody, anti-mitochondria antibody and anti-smooth muscle antibody. The distribution of samples and analysis of the testing results was double-blinded. Result The correct rate of ANA, anti-ENA antibody, anti-dsDNA antibody, anti-mitochondria antibody and anti-smooth muscle antibody were 47%, 70%, 46%, 95%and 40% respectively. Conclusion The overall results are not optimistic. The quality of detecting kits is not all good, neither level of technicians. This condition should be changed by selecting better methods and improving understanding.
出处 《中华风湿病学杂志》 CAS CSCD 2004年第7期387-390,共4页 Chinese Journal of Rheumatology
关键词 医院 自身抗体 检测 质量控制 实验室诊断 Antibodies Quality control Analysis
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参考文献4

  • 1Arthur K,Russell T,John R,et al.Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens.Arch of Pathol Lab Med.,124: 7-81.
  • 2Hoffman IE,Peene I,Veys EM,et al.Detection of specific antinuclear reactivities in patients with negative anti-nuclear antibody immunofluorescence screening tests.Clin Chem,2002,48: 2163-2171.
  • 3McMillan SA,Fay AC.Evaluation of five commercial kits to detect dsDNA antibodies.J Clin Pathol,1988,41: 1223-1228.
  • 4Bridges AJ,Lorden TE,Havighurst TC,et al.Autoantibody testing for connective tissue diseases.Comparison of immunodiffusion,immunoblot,and enzyme immunoassay.Am J Clin Pathol 1997,108: 406-410.

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