摘要
目的 :探讨不可分型流感嗜血杆菌 (NTHi)与气液界面无血清培养原代兔气管黏膜上皮细胞的相互作用。 方法 :利用低温酶消化法分离兔气管黏膜上皮细胞 ,无血清培养液及胶原覆盖膜形成的气液界面培养 ,使上皮细胞分化成假复层黏膜纤毛上皮细胞 ,然后加入 NTHi感染上皮细胞 ,通过扫描电镜 (SEM)和透射电镜 (TEM)观察其形态结构变化。 结果 :NTHi感染 2 4h后 ,SEM示气管黏膜上皮细胞表面结构破坏 ,90 %细胞凋亡或死亡 ,纤毛倒伏、断裂 ,细菌与非纤毛上皮细胞连接 ;TEM示细菌黏附在细胞表面 ,细胞表面有较多微绒毛 ,伴微绒毛延伸或伪足包绕细菌 ,将其吞噬胞内。结论 :NTHi黏附在上皮细胞表面 ,上皮细胞通过伪足形成和微绒毛延伸包绕细菌 ,将细菌吞噬在胞内 ;NTHi对上皮细胞有毒性作用 。
Objective:To explore the effect of nontypeable Haemophilus influenzaen(NTHi)strain ATCC49247on pri-mary rabbit tracheal epithelial(TE)cells.Methods:TE cells were isolated with low temperature protease digestion and cul-tured on collagen gel-coated membranes at an air-liquid interface in serum-free medium.Under these conditions,TE cells were proliferated and differentiated into a pseudostratified mucociliary epithelium,which were infected by NTHi.Morphologic changes of the cells were examined by scanning electron microscopy(SEM)and transmission electron microscopy(TEM)after 24h.Results:SEM showed that bacteria adhered to non-ciliated cells;death or apoptosis occurred in90%of TE cells and cil-iaries were broken.TEM showed NTHi adhered to the cell surface on which there were many microvillus.Lamellipodia and microvilli surrounded bacteria within vacuoles of airway cells.Conclusion:NTHi can attach to non-ciliated cells,the latter de-vours the bacteria by lamellipodia and microvilli.NTHi is toxic to TE cells,resulting in the death or apoptosis of TE cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2004年第7期769-772,共4页
Academic Journal of Second Military Medical University
基金
渤林格殷格翰 COPD资助项目 ( 980 3 )
